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石墨烯多孔钛合金支架中外泌体促进脂肪干细胞成骨分化的研究

Study on Exosomes Promoting the Osteogenic Differentiation of ADSCs in Graphene Porous Titanium Alloy Scaffolds.

作者信息

Sun Xu, Yang Shude, Tong Shuang, Guo Shu

机构信息

Department of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China.

出版信息

Front Bioeng Biotechnol. 2022 Jun 6;10:905511. doi: 10.3389/fbioe.2022.905511. eCollection 2022.

DOI:10.3389/fbioe.2022.905511
PMID:35733528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9207277/
Abstract

Titanium and titanium alloys (TiAlV and Ti) have been widely used in bone tissue engineering to repair maxillofacial bone defects caused by traumas and tumors. However, such materials are also bio-inert, which does not match the elastic modulus of bone. Therefore, different surface modifications have been proposed for clinical application. Based on the use of traditional titanium alloy in the field of bone repair defects, we prepared a compound Gr-Ti scaffold with ADSC-derived Exos. The results showed that Gr-Ti scaffolds have low toxicity and good biocompatibility, which can promote the adhesion and osteogenic differentiation of ADSCs. Exos played a role in promoting osteogenic differentiation of ADSCs: the mRNA levels of , , and in the Gr-Ti/Exos group were significantly higher than those in the Gr-Ti group, which process related to the Wnt signaling pathway. Gr-Ti scaffolds with ADSCs and ADSC-derived Exos successfully repaired rabbit mandibular defects. The bone mineral density and the bending strength of the Gr-Ti/Exos group was significantly higher than that of the Gr-Ti group. This study provides a theoretical basis for the research and development of new clinical bone repair materials.

摘要

钛及钛合金（TiAlV和Ti）已广泛应用于骨组织工程，以修复由创伤和肿瘤引起的颌面骨缺损。然而，这类材料具有生物惰性，与骨的弹性模量不匹配。因此，人们提出了不同的表面改性方法用于临床应用。基于传统钛合金在骨修复缺损领域的应用，我们制备了一种搭载脂肪干细胞来源外泌体的复合Gr-Ti支架。结果表明，Gr-Ti支架具有低毒性和良好的生物相容性，能够促进脂肪干细胞的黏附和成骨分化。外泌体在促进脂肪干细胞成骨分化中发挥作用：Gr-Ti/外泌体组中、和的mRNA水平显著高于Gr-Ti组，该过程与Wnt信号通路有关。搭载脂肪干细胞和脂肪干细胞来源外泌体的Gr-Ti支架成功修复了兔下颌骨缺损。Gr-Ti/外泌体组的骨密度和弯曲强度显著高于Gr-Ti组。本研究为新型临床骨修复材料的研发提供了理论依据。

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