Department of Neurosurgery, Lenox Hill Hospital/Donald, Barbara Zucker School of Medicine at Hofstra, New York, NY, USA.
Department of Pathology, Lenox Hill Hospital/Donald, Barbara Zucker School of Medicine at Hofstra, New York, NY, USA.
J Neurooncol. 2022 Sep;159(2):369-375. doi: 10.1007/s11060-022-04071-y. Epub 2022 Jun 28.
Stimulated Raman histology (SRH) offers efficient and accurate intraoperative neuropathological tissue analysis without procedural alteration to the diagnostic specimen. However, there are limited data demonstrating one-to-one tissue comparisons between SRH and traditional frozen sectioning. This study explores the non-inferiority of SRH as compared to frozen section on the same piece of tissue in neurosurgical patients.
Tissue was collected over a 1-month period from 18 patients who underwent resection of central nervous system lesions. SRH and frozen section analyses were compared for diagnostic capabilities as well as assessed for quality and condition of tissue via a survey completed by pathologists.
SRH was sufficient for diagnosis in 78% of specimens as compared to 94% of specimens by frozen section of the same specimen. A Fisher's exact test determined there was no significant difference in diagnostic capability between the two groups. Additionally, both quality of SRH and condition of tissue after SRH were deemed to be non-inferior to frozen section.
This study provides further evidence for the non-inferiority of SRH techniques. It is also the first study to demonstrate SRH accuracy using one-to-one tissue analysis in neuropathological specimens.
受激拉曼组织学（SRH）可在不改变诊断标本的手术过程的情况下，提供高效、准确的术中神经病理学组织分析。然而，目前仅有有限的数据能够证明 SRH 与传统冷冻切片之间的组织一一对应比较。本研究旨在探索 SRH 在神经外科患者中与冷冻切片相比的非劣效性。
在 1 个月的时间内，从 18 名接受中枢神经系统病变切除术的患者中收集组织。对 SRH 和冷冻切片分析进行比较，以评估其诊断能力，并通过病理学家完成的调查评估组织的质量和状况。
SRH 在 78%的标本中足以做出诊断，而同一标本的冷冻切片为 94%。Fisher 精确检验确定两组之间的诊断能力无显著差异。此外，SRH 的质量和 SRH 后组织的状况均被认为与冷冻切片相当。
本研究进一步证明了 SRH 技术的非劣效性。这也是第一项使用神经病理学标本的组织一一对应分析来证明 SRH 准确性的研究。
