Department Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA.
School of Kinesiology, Auburn University, Auburn, AL, USA.
J Acupunct Meridian Stud. 2022 Feb 28;15(1):12-24. doi: 10.51507/j.jams.2022.15.1.12.
Intra-organic bone marrow node is predicted to be a part of the primo vascular system that plays a critical role in hematopoiesis and generation and regeneration of other cells. Two models of cell regeneration were suggested, one involving DNA synthesis and the other pertaining to DNA recycling. Objectives: The aim of this work is to extract a primo node from bone marrow, characterize its structure, understand its biochemistry and cell composition, and suggest a cell regeneration mechanism.
Primo nodes were sampled from segmented halves of the rat femur. We used immunohistochemistry and high-resolution fluorescent microscopy to analyze 1200 samples obtained from 42 rats and 190 primo nodes.
Primo nodes in the bone marrow have an oval or round structure of about one millimeter in diameter, which is encompassed by a fine capsule, having incoming and outgoing vessels filled with the extracellular matrix and hematopoietic, mesenchymal, endothelial stem cells, as well as cells of the megakaryocyte family found in other primo nodes.
Our findings imply that bone marrow nodes are intra-organic primo vascular nodes, and they provide ways and approaches for further investigation. Bone marrow nodes are simple to examine ex vivo in a variety of environments to assess cell regeneration mechanisms, wound healing, and organism rejuvenation and lifespan. Further research into these and other intra-organic nodes in animals and humans could lead to new regenerative medicine and longevity strategies that have yet to be discovered.
骨髓内的骨节点被预测为原始血管系统的一部分，在造血以及其他细胞的生成和再生中起着关键作用。提出了两种细胞再生模型，一种涉及 DNA 合成，另一种与 DNA 回收有关。目的：本工作的目的是从骨髓中提取原始节点，对其结构进行特征描述，了解其生物化学和细胞组成，并提出一种细胞再生机制。
从大鼠股骨的分段半部分中采集原始节点。我们使用免疫组织化学和高分辨率荧光显微镜分析了来自 42 只大鼠和 190 个原始节点的 1200 个样本。
骨髓中的原始节点具有约一毫米直径的椭圆形或圆形结构，被精细的囊包围，有输入和输出血管，充满细胞外基质和造血、间充质、内皮干细胞，以及在其他原始节点中发现的巨核细胞家族的细胞。
我们的发现表明骨髓节点是有机体内的原始血管节点，并为进一步研究提供了途径和方法。骨髓节点在各种环境中很容易进行离体检查，以评估细胞再生机制、伤口愈合以及机体的恢复和寿命。对动物和人类这些和其他内在器官节点的进一步研究可能会带来新的再生医学和长寿策略，这些策略尚未被发现。
