Nance S J, Garratty G
Am J Clin Pathol. 1987 May;87(5):633-5. doi: 10.1093/ajcp/87.5.633.
A new technic using polyethylene glycol (PEG) was developed to detect and enhance weak red blood cell/antibody reactions. Twenty percent PEG of 4,000 molecular weight was found to be optimal. Weakly reactive antibodies (n = 25) were tested by PEG, Polybrene, and low ionic strength saline (LISS); 64% were strongest in PEG, 28% reacted equally in PEG as in Polybrene or LISS, 8% reacted weaker in PEG than in Polybrene or LISS. Stronger antibodies (n = 11) were titrated and compared in the three technics; in 10 of 11 titrations, PEG was better or equal to Polybrene and LISS. The false positive rate with the use of PEG and anti-IgG was 1.5% with the use of random sera. Sera from patients (n = 24) with hemolytic transfusion reactions and no detectable antibody by routine technics were tested; two sera had specific antibodies by the PEG technic. This new technic should be a valuable aid in the detection and identification of weak antibodies.
一种使用聚乙二醇（PEG）的新技术被开发出来，用于检测和增强微弱的红细胞/抗体反应。发现4000分子量的20% PEG是最佳的。用PEG、聚凝胺和低离子强度盐水（LISS）对25份弱反应性抗体进行了检测；64%在PEG中反应最强，28%在PEG中的反应与在聚凝胺或LISS中的反应相同，8%在PEG中的反应比在聚凝胺或LISS中的反应弱。对11份强反应性抗体进行了三种技术的滴定和比较；在11次滴定中的10次中，PEG优于或等同于聚凝胺和LISS。使用随机血清时，使用PEG和抗IgG的假阳性率为1.5%。对24例有溶血性输血反应且常规技术未检测到抗体的患者血清进行了检测；有两份血清通过PEG技术检测出特异性抗体。这项新技术在检测和鉴定弱抗体方面应是一项有价值的辅助手段。
