State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center, Collaborative Innovation Center of Chemical Science and Engineering, College of Chemistry, Nankai University, 300071, Tianjin, P. R. China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, 410082, Changsha, P. R. China.
Nat Commun. 2022 Jul 9;13(1):3987. doi: 10.1038/s41467-022-31710-9.
The development of potent strigolactone (SL) agonists as suicidal germination inducers could be a useful strategy for controlling root parasitic weeds, but uncertainty about the SL perception mechanism impedes real progress. Here we describe small-molecule agonists that efficiently stimulate Phelipanchce aegyptiaca, and Striga hermonthica, germination in concentrations as low as 10 to 10 M. We show that full efficiency of synthetic SL agonists in triggering signaling through the Striga SL receptor, ShHTL7, depends on the receptor-catalyzed hydrolytic reaction of the agonists. Additionally, we reveal that the stereochemistry of synthetic SL analogs affects the hydrolytic ability of ShHTL7 by influencing the probability of the privileged conformations of ShHTL7. Importantly, an alternative ShHTL7-mediated hydrolysis mechanism, proceeding via nucleophilic attack of the NE2 atom of H246 to the 2'C of the D-ring, is reported. Together, our findings provide insight into SL hydrolysis and structure-perception mechanisms, and potent suicide germination stimulants, which would contribute to the elimination of the noxious parasitic weeds.
作为自杀萌发诱导剂的强效 Strigolactone (SL) 激动剂的开发可能是控制根寄生杂草的有效策略，但对 SL 感知机制的不确定性阻碍了真正的进展。在这里，我们描述了能够以低至 10 到 10 M 的浓度有效刺激 Phelipanchce aegyptiaca 和 Striga hermonthica 萌发的小分子激动剂。我们表明，合成 SL 激动剂在通过 Striga SL 受体 ShHTL7 触发信号转导中的全部效率取决于受体催化的激动剂的水解反应。此外，我们揭示了合成 SL 类似物的立体化学通过影响 ShHTL7 的特权构象的概率来影响 ShHTL7 的水解能力。重要的是，报道了替代的 ShHTL7 介导的水解机制，该机制通过 H246 的 NE2 原子对 D-环的 2'C 的亲核攻击进行。总之，我们的发现为 SL 水解和结构感知机制以及有效的自杀萌发刺激剂提供了深入的了解，这将有助于消除有害的寄生杂草。
