Wootton R, Baskerville P, Turner P, Insell M, Shaikh M, La Ville A, Quiney J, Browse N L, Lewis B
Clin Phys Physiol Meas. 1987 Feb;8(1):65-74. doi: 10.1088/0143-0815/8/1/005.
A new method is proposed for measuring in vivo the rates of transfer of plasma lipoprotein into and out of arterial intima in man. The technique requires the injection of a lipoprotein which can be labelled with two different tracers, followed by a single arterial sample during elective surgery. The feasibility of the method was tested by computer simulation and it was found that measurement uncertainties of the order of those likely in practice produced uncertainties of 7% SD in the calculated influx and 16% SD in the calculated efflux rates. The principal assumption, that lipoprotein in arterial intima can be modelled as a single compartment system, was tested in a preliminary study in an anaesthetised dog: reasonable evidence of monocompartmental behaviour was obtained. The new method was used in a preliminary study of three patients undergoing elective arterial surgery. Low density lipoprotein (LDL) labelled with 125I was injected about 24 h before surgery, followed by 131I-labelled LDL 4 to 6 h before surgery. The radioactivities of washed extracts of arterial intima were then measured in a gamma counter. LDL flux rates were higher in atherosclerotic specimens than in less seriously diseased or normal tissue, and were highest in the most hyperlipidaemic patient.
提出了一种在人体中体内测量血浆脂蛋白进出动脉内膜转移速率的新方法。该技术需要注射一种可用两种不同示踪剂标记的脂蛋白，然后在择期手术期间采集单个动脉样本。通过计算机模拟测试了该方法的可行性，发现实际可能出现的测量不确定度在计算的流入量中产生了7%标准差的不确定度，在计算的流出速率中产生了16%标准差的不确定度。主要假设，即动脉内膜中的脂蛋白可被建模为单室系统，在一项对麻醉犬的初步研究中进行了测试：获得了单室行为的合理证据。该新方法用于对三名接受择期动脉手术的患者进行的初步研究。在手术前约24小时注射用125I标记的低密度脂蛋白（LDL），然后在手术前4至6小时注射131I标记的LDL。然后在γ计数器中测量动脉内膜洗涤提取物的放射性。动脉粥样硬化标本中的LDL通量率高于病情较轻或正常的组织，在血脂最高的患者中LDL通量率最高。
