Host-Microbe Interactomics Group, Department of Animal Sciences, Wageningen University & Research, P.O. Box 338, 6700 AH, Wageningen, The Netherlands.
Adaptation Physiology Group, Department of Animal Sciences, Wageningen University & Research, P.O. Box 338, 6700 AH, Wageningen, The Netherlands.
Sci Rep. 2022 Jul 19;12(1):12322. doi: 10.1038/s41598-022-16259-3.
It is increasingly recognised that the microbes residing in the gastrointestinal tract can influence brain physiology and behaviour, via the microbiota-gut-brain axis. Here, we made a first explorative evaluation at the association between the gut microbiota and behaviour in suckling piglets. 16S microbiota profiling information was obtained from two independent replicate experiments at 2 and 4 weeks of age. Piglets underwent a backtest to assess their personality or coping style at 2 weeks of age, and were subjected to a combined open field and novel object test at 3.5 weeks of age, recording anxiety-related and exploratory behaviour. The number of squeals vocalised during the open field test was associated with microbial groups such as Coprococcus 3 and CAG-873, whereas in the novel object test, explorative behaviour was significantly associated with microbial genera like Atopobium and Prevotella. Overall, this study explores the microbiota-behavioural relation by employing multivariate analysis and exemplifies the importance of individualised analyses when evaluating such relationships.
越来越多的人认识到，肠道中的微生物可以通过微生物群-肠-脑轴影响大脑的生理和行为。在这里，我们首次探索了哺乳期仔猪肠道微生物群与行为之间的关系。在 2 周和 4 周龄时进行了两个独立的重复实验，获得了 16S 微生物组分析信息。在 2 周龄时，仔猪进行了回溯测试以评估其个性或应对方式，在 3.5 周龄时进行了组合式开放场和新物体测试，记录了焦虑相关和探索行为。在开放场测试中发出的尖叫声数量与 Coprococcus 3 和 CAG-873 等微生物群有关，而在新物体测试中，探索性行为与 Atopobium 和 Prevotella 等微生物属显著相关。总的来说，这项研究通过采用多变量分析探索了微生物群-行为关系，并说明了在评估这种关系时进行个体化分析的重要性。
