Wang Shuntao, Zeng Ning, Zhang Qi, Chen Mingzhu, Huang Qinqin
Department of Molecular Pathology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Front Oncol. 2022 Jul 4;12:888855. doi: 10.3389/fonc.2022.888855. eCollection 2022.
Sonosensitizer-mediated sonodynamic therapy (SDT) has emerged as a promising anti-tumor strategy. However, this strategy of continuous oxygen consumption further exacerbates the hypoxic tumor microenvironment, which limits its therapeutic efficacy. In this study, we designed a multifunctional hydrogel (PB+Ce6@Hy) that simultaneously co-delivers nanozyme prussian blue (PB) and sonosensitizer chlorin e6 (Ce6) for the realization of photothermal therapy (PTT) and enhanced SDT. When the hydrogel reaches the tumor tissue through local injection, the 808 nm laser can induce the hydrogel to warm up and soften, thereby triggering the release of PB and Ce6. PB can interact with endogenous HO and generate sufficient oxygen to promote the Ce6-mediated SDT effect. Besides, due to the good encapsulation ability of the hydrogel, the nanomaterials can be released in a controlled manner by changing laser parameter, irradiation time, etc. The experimental results show that the PB+Ce6@Hy system we developed can generate a large amount of reactive oxygen species (ROS), which can be combined with the photothermal effect to kill tumor cells, as a result, tumor proliferation has been adequately inhibited. This combined PTT/SDT dynamic strategy provides a new perspective for Ce6-induced cancer therapy, showing great potential for clinical application.
声敏剂介导的声动力疗法（SDT）已成为一种很有前景的抗肿瘤策略。然而，这种持续消耗氧气的策略会进一步加剧肿瘤缺氧微环境，从而限制其治疗效果。在本研究中，我们设计了一种多功能水凝胶（PB+Ce6@Hy），它能同时共递送纳米酶普鲁士蓝（PB）和声敏剂二氢卟吩e6（Ce6），以实现光热疗法（PTT）并增强声动力疗法。当水凝胶通过局部注射到达肿瘤组织时，808 nm激光可诱导水凝胶升温并软化，从而触发PB和Ce6的释放。PB可与内源性过氧化氢相互作用并产生足够的氧气，以促进Ce6介导的声动力疗法效果。此外，由于水凝胶具有良好的包封能力，通过改变激光参数、照射时间等，纳米材料可以可控方式释放。实验结果表明，我们开发的PB+Ce6@Hy系统可产生大量活性氧（ROS），其可与光热效应相结合来杀死肿瘤细胞，结果肿瘤增殖得到了充分抑制。这种联合PTT/SDT动态策略为Ce6诱导的癌症治疗提供了新的视角，显示出巨大的临床应用潜力。
