Gaertner D J, Boschert K R, Schoeb T R
Lab Anim Sci. 1987 Feb;37(1):80-3.
To assess tissue damage resulting from intramuscular injection of mixtures of ketamine and xylazine, 48 hamsters were given 100, 150 or 200 mg/kg ketamine and 10 mg/kg xylazine in one hind leg and an equal volume of sterile physiologic saline in the other leg. Four hamsters from each group were killed 1, 3, 7 and 14 days after injection and the tissues at the injection sites were examined. There was grossly apparent muscle necrosis in most of the ketamine-xylazine injected legs. By light microscopy, 47 of 48 legs injected with ketamine-xylazine had moderate to extensive muscle necrosis with an acute to chronic inflammatory response, depending on the time elapsed since injection. Microscopic slides of the injection sites were coded, randomized and scored for severity of muscle lesions. Lesion scores for ketamine-xylazine injected legs were significantly higher than controls at all post-injection times. These findings indicate that intramuscular injection of ketamine with xylazine can cause extensive muscle necrosis in hamsters and should not be used for anesthesia in survival procedures.
为评估肌肉注射氯胺酮和赛拉嗪混合物所导致的组织损伤，48只仓鼠在一条后腿注射100、150或200mg/kg氯胺酮及10mg/kg赛拉嗪，另一条后腿注射等体积的无菌生理盐水。每组4只仓鼠在注射后1、3、7和14天处死，检查注射部位的组织。在大多数注射氯胺酮-赛拉嗪的后腿中，肉眼可见明显的肌肉坏死。通过光学显微镜观察，48只注射氯胺酮-赛拉嗪的腿中有47只出现中度至广泛的肌肉坏死，并伴有急性至慢性炎症反应，这取决于注射后的时间。对注射部位的显微镜载玻片进行编码、随机分组，并对肌肉损伤的严重程度进行评分。在所有注射后的时间点，注射氯胺酮-赛拉嗪的腿的损伤评分均显著高于对照组。这些发现表明，肌肉注射氯胺酮和赛拉嗪可导致仓鼠广泛的肌肉坏死，不应在存活实验中用于麻醉。
