Smiler K L, Stein S, Hrapkiewicz K L, Hiben J R
Biomedical Science Department, General Motors Research Laboratories, Warren, MI 48090-9058.
Lab Anim Sci. 1990 Jan;40(1):60-4.
Ketamine-xylazine is a widely accepted anesthetic combination for laboratory animals. Although frequently recommended for administration by intramuscular (IM) or intraperitoneal (IP) routes, the potential for tissue damage following either route of administration in the rat has not been investigated. This study evaluated tissue damage after IM use at two doses in Fischer 344 and Sprague-Dawley rats. Tissue reactions following IP injections of ketamine-xylazine were compared to lesions produced by IM injections in animals euthanatized on 1, 3 and 14 days post-injection. Results showed muscle necrosis present in nearly all ketamine-xylazine injected limbs. Intraperitoneal injections produced no significant lesions in the peritoneal cavity when careful IP injection techniques were used. Ketamine-xylazine should not be administered by the IM route for survival procedures in these two widely used strains of rats.
氯胺酮-甲苯噻嗪是一种广泛用于实验动物的麻醉合剂。尽管经常推荐通过肌肉注射（IM）或腹腔注射（IP）途径给药，但尚未研究这两种给药途径在大鼠中造成组织损伤的可能性。本研究评估了在Fischer 344和Sprague-Dawley大鼠中以两种剂量进行肌肉注射后造成的组织损伤。将腹腔注射氯胺酮-甲苯噻嗪后的组织反应与在注射后第1、3和14天实施安乐死的动物中肌肉注射造成的损伤进行比较。结果显示，几乎所有注射氯胺酮-甲苯噻嗪的肢体均出现肌肉坏死。当采用谨慎的腹腔注射技术时，腹腔注射在腹腔内未产生明显病变。对于这两种广泛使用的大鼠品系，在生存实验中不应通过肌肉注射途径给予氯胺酮-甲苯噻嗪。
