Center for Stem Cell and Regenerative Medicine, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.
Liangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, Zhejiang, 311121, China.
Nat Commun. 2022 Jul 22;13(1):4228. doi: 10.1038/s41467-022-31879-z.
The Mexican axolotl (Ambystoma mexicanum) is a well-established tetrapod model for regeneration and developmental studies. Remarkably, neotenic axolotls may undergo metamorphosis, a process that triggers many dramatic changes in diverse organs, accompanied by gradually decline of their regeneration capacity and lifespan. However, the molecular regulation and cellular changes in neotenic and metamorphosed axolotls are still poorly investigated. Here, we develop a single-cell sequencing method based on combinatorial hybridization to generate a tissue-based transcriptomic landscape of the neotenic and metamorphosed axolotls. We perform gene expression profiling of over 1 million single cells across 19 tissues to construct the first adult axolotl cell landscape. Comparison of single-cell transcriptomes between the tissues of neotenic and metamorphosed axolotls reveal the heterogeneity of non-immune parenchymal cells in different tissues and established their regulatory network. Furthermore, we describe dynamic gene expression patterns during limb development in neotenic axolotls. This system-level single-cell analysis of molecular characteristics in neotenic and metamorphosed axolotls, serves as a resource to explore the molecular identity of the axolotl and facilitates better understanding of metamorphosis.
墨西哥钝口螈（Ambystoma mexicanum）是一种成熟的四足动物再生和发育研究模型。值得注意的是，幼态性墨西哥钝口螈可能会经历变态，这一过程会引发多种不同器官的剧烈变化，同时伴随着其再生能力和寿命的逐渐下降。然而，幼态和变态墨西哥钝口螈的分子调控和细胞变化仍未得到充分研究。在这里，我们开发了一种基于组合杂交的单细胞测序方法，生成了幼态和变态墨西哥钝口螈的基于组织的转录组图谱。我们对 19 种组织中的超过 100 万个单细胞进行了基因表达谱分析，构建了第一个成年墨西哥钝口螈细胞图谱。对幼态和变态墨西哥钝口螈组织的单细胞转录组进行比较，揭示了不同组织中非免疫实质细胞的异质性，并建立了它们的调控网络。此外，我们描述了幼态墨西哥钝口螈肢体发育过程中的动态基因表达模式。这种对幼态和变态墨西哥钝口螈分子特征的系统水平的单细胞分析，为探索墨西哥钝口螈的分子特征提供了资源，并有助于更好地理解变态。
