Al-Ansari Dana E, Al-Badr Mashael, Zakaria Zain Z, Mohamed Nura Adam, Nasrallah Gheyath K, Yalcin Huseyin C, Abou-Saleh Haissam
Biological Science Program, Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Doha, Qatar.
Division of Biological and Biomedical Sciences, College of Health and Life Sciences, Hamad Bin Khalifa University, Qatar Foundation, Doha, Qatar.
Toxicol Rep. 2022 Apr 20;9:951-960. doi: 10.1016/j.toxrep.2022.04.016. eCollection 2022.
Metal-Organic Framework MIL-89 nanoparticles garnered remarkable attention for their widespread use in technological applications. However, the impact of these nanomaterials on human and environmental health is still limited, and concerns regarding the potential risk of exposure during manipulation is constantly rising. Therefore, the extensive use of nanomaterials in the medical field necessitates a comprehensive assessment of their safety and interaction with different tissues of the body system. In this study, we evaluated the systemic toxicity of nanoMIL-89 using Zebrafish embryos as a model system to determine the acute developmental effect. Zebrafish embryos were exposed to a range of nanoMIL-89 concentrations (1 - 300 µM) at 4 h post-fertilization (hpf) for up to 120 hpf. The viability and hatching rate were evaluated at 24-72 hpf, whereas the cardiac function was assessed at 72 and 96 hpf, and the neurodevelopment and hepatic steatosis at 120 hpf. Our study shows that nanoMIL-89 exerted no developmental toxicity on zebrafish embryos at low concentrations (1-10 µM). However, the hatching time and heart development were affected at high concentrations of nanoMIL-89 (> 30 µM Our findings add novel information into the available data about the in vivo toxicity of nanoMIL-89 and demonstrate its innocuity and safe use in biological, environmental, and medical applications.
金属有机框架MIL-89纳米颗粒因其在技术应用中的广泛使用而备受关注。然而，这些纳米材料对人类和环境健康的影响仍然有限，并且在操作过程中对潜在暴露风险的担忧也在不断增加。因此，纳米材料在医学领域的广泛应用需要对其安全性以及与人体系统不同组织的相互作用进行全面评估。在本研究中，我们以斑马鱼胚胎作为模型系统评估了纳米MIL-89的全身毒性，以确定其急性发育效应。在受精后4小时（hpf），将斑马鱼胚胎暴露于一系列纳米MIL-89浓度（1 - 300 μM）下，长达120 hpf。在24 - 72 hpf评估活力和孵化率，而在72和96 hpf评估心脏功能，在120 hpf评估神经发育和肝脂肪变性。我们的研究表明，低浓度（1 - 10 μM）的纳米MIL-89对斑马鱼胚胎没有发育毒性。然而，高浓度（> 30 μM）的纳米MIL-89会影响孵化时间和心脏发育。我们的研究结果为现有的关于纳米MIL-89体内毒性的数据增添了新信息，并证明了其在生物、环境和医学应用中的无害性和安全使用。
