Agriculture College, Ningxia University, Yinchuan, Ning'xia, China.
Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Tai'an , 271018, China.
Arch Microbiol. 2022 Jul 25;204(8):521. doi: 10.1007/s00203-022-03024-3.
The application of nematicidal microorganisms and their virulence factors provides more opportunities to control root-knot nematodes. Bacillus altitudinis AMCC 1040, previously isolated from suppressive soils, showed significant nematicidal activity, and in this study, nematicidal substances produced by Bacillus altitudinis AMCC 1040 were investigated. The results of the basic properties of active substances showed that these compounds have good thermal stability and passage, are resistant to acidic environment and sensitive to alkaline conditions. Further analysis showed that it is a volatile component. Using HS-SPME-GC/MS, the volatile compounds produced by Bacillus altitudinis AMCC 1040 were identified and grouped into four major categories: ethers, alcohols, ketone, and organic acids, comprising a total of eight molecules. Six of them possess nematicidal activities, including 2,3-butanedione, acetic acid, 2-isopropoxy ethylamine, 3-methylbutyric acid, 2-methylbutyric acid and octanoic acid. Our results further our understanding of the effects of Bacillus altitudinis and its nematicidal metabolites on the management of Meloidogyne incognita and may help in finding less toxic nematicides to control root knot nematodes.
杀线虫微生物及其毒力因子的应用为防治根结线虫提供了更多的机会。先前从抑病土壤中分离到的高山芽孢杆菌 AMCC 1040 表现出显著的杀线虫活性，本研究对高山芽孢杆菌 AMCC 1040 产生的杀线虫物质进行了研究。活性物质基本性质的结果表明，这些化合物具有良好的热稳定性和通透性，能耐受酸性环境，对碱性条件敏感。进一步分析表明，它是一种挥发性成分。采用 HS-SPME-GC/MS 对高山芽孢杆菌 AMCC 1040 产生的挥发性化合物进行了鉴定和分类，分为醚类、醇类、酮类和有机酸四大类，共 8 种分子。其中 6 种具有杀线虫活性，包括 2,3-丁二酮、乙酸、2-异丙氧基乙胺、3-甲基丁酸、2-甲基丁酸和辛酸。我们的研究结果进一步了解了高山芽孢杆菌及其杀线虫代谢物对防治南方根结线虫的影响，并可能有助于寻找毒性更低的杀线虫剂来防治根结线虫。
