Guo Yatao, Zhai Yao, Zhang Zhao, Li Daixi, Wang Zhanwei, Li Jingquan, He Zilong, Hu Songnian, Kang Yu, Gao Zhancheng
Department of Respiratory and Critical Care Medicine, Peking University People's Hospital, Beijing, China.
University of Technology Sydney, Ultimo, NSW, Australia.
Front Microbiol. 2018 Oct 9;9:2428. doi: 10.3389/fmicb.2018.02428. eCollection 2018.
Bacterial isolate X39 was isolated from a community-acquired pneumonia patient in Beijing, China. A phylogenetic tree based on genes and average nucleotide identity data confirmed that isolate X39 belonged to . The genome of X39 contained one circular chromosome and nine plasmids. Comparative genomic analyses with other isolates revealed that X39 contained the most unique genes. Of these unique genes, many were prophages and transposases. Many virulence factors were shared between X39 and F1. The pathogenicity of X39 was compared with that of F1 in an abdominal infection model. The results indicated that X39 was less virulent than typical clinical F1. The genome of X39 also contained some genes involved in plant colonization, nitrogen fixation, and defense against oxidative stress. GFP-labeled X39 could colonize maize as an endophytic bacterium. We concluded that X39 was a kingdom-crossing strain.
细菌分离株X39是从中国北京一名社区获得性肺炎患者中分离出来的。基于基因和平均核苷酸同一性数据构建的系统发育树证实分离株X39属于……。X39的基因组包含一条环状染色体和九个质粒。与其他……分离株进行的比较基因组分析表明，X39含有最多的独特基因。在这些独特基因中，许多是原噬菌体和转座酶。X39和F1之间共享许多毒力因子。在腹部感染模型中比较了X39和F1的致病性。结果表明，X39的毒力低于典型临床分离株F1。X39的基因组还包含一些参与植物定殖、固氮和抗氧化应激防御的基因。绿色荧光蛋白标记的X39可以作为内生细菌定殖在玉米中。我们得出结论，X39是一种跨界菌株。
