Duan Mingzheng, Lu Meiling, Lu Jia, Yang Wenjing, Li Bo, Ma Li, Wang Lingqiang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, 100 Daxue Rd., Nanning 530004, China.
J Fungi (Basel). 2022 Jun 28;8(7):680. doi: 10.3390/jof8070680.
A unique ecological landscape distributed in the Mongolian Plateau, called fairy rings, caused by the growth of the fungus (LM) in the soil could promote plant growth without fertilization. Therefore, this landscape can alleviate fertilizer use and has excellent value for agricultural production. The previous studies only investigated several parameters of the fairy rings, such as soil microbial diversity and some soil chemical properties, thus conclusions based on the studies on fairy rings lack comprehension. Therefore, the present study systematically investigated the chemical properties, metabolome, and metabarcoding of LM-transformed soil. We analyzed fairy ring soils from DARK (FR) and OUT (CK) zone correlated growth promotion with ten soil chemical properties, including N, nitrate-N, inorganic-P, cellulose, available boron, available sulfur, Fe, Mn, Zn, and Cu, which were identified as important markers to screen fairy ring landscapes. Metabolomics showed that the accumulation of 17 carbohydrate-dominated metabolites was closely associated with plant growth promotion. Finally, metabarcoding detected fungi as the main components affecting soil conversion. Among the various fungi at the family level, Lasiosphaeriaceae, unidentified_Auriculariales_sp, and Herpotrichiellaceae were markers to screen fairy ring. Our study is novel and systematically reveals the fairy ring soil ecology and lists the key factors promoting plant growth. These findings lay a theoretical foundation for developing the fairy ring landscape in an agricultural system.
分布在蒙古高原的一种独特生态景观——仙人圈，是由土壤中真菌（LM）生长形成的，它能在不施肥的情况下促进植物生长。因此，这种景观可以减少化肥使用，对农业生产具有极高价值。以往研究仅调查了仙人圈的几个参数，如土壤微生物多样性和一些土壤化学性质，因此基于仙人圈研究得出的结论缺乏全面性。所以，本研究系统地调查了LM转化土壤的化学性质、代谢组和宏条形码。我们分析了来自黑暗（FR）区和外围（CK）区与生长促进相关的仙人圈土壤的十种土壤化学性质，包括氮、硝态氮、无机磷、纤维素、有效硼、有效硫、铁、锰、锌和铜，这些被确定为筛选仙人圈景观的重要指标。代谢组学表明，17种以碳水化合物为主的代谢物的积累与植物生长促进密切相关。最后，宏条形码检测到真菌是影响土壤转化的主要成分。在科级水平的各种真菌中，毛球壳菌科、未鉴定的木耳目物种和刺壳孢科是筛选仙人圈的指标。我们的研究具有创新性，系统地揭示了仙人圈土壤生态，并列出了促进植物生长的关键因素。这些发现为在农业系统中开发仙人圈景观奠定了理论基础。
