Laboratório de Pesquisas em Virologia [LPV], Faculdade de Medicina de São José do Rio Preto [FAMERP], Avenida Brigadeiro Faria Lima, 5544, Vila São Jose, São José do Rio Preto 15090-000, SP, Brazil.
Laboratório de Imunologia Celular e Molecular (LICM), Avenida Augusto de Lima, 1715, Centro, Belo Horizonte 30190-002, MG, Brazil.
Viruses. 2022 Jul 7;14(7):1488. doi: 10.3390/v14071488.
Arbovirus infections are increasingly important causes of disease, whose spectrum of neurological manifestations are not fully known. This study sought to retrospectively assess the incidence of arboviruses in cerebrospinal fluid samples of patients with neurological symptoms to inform diagnosis of central and peripheral nervous system disorders. A total of 255 cerebrospinal fluid (CSF) samples collected from January 2016 to December 2017 were tested for dengue virus (DENV 1-4), Zika virus (ZIKV), and Chikungunya virus (CHIKV) in addition to other neurotropic arboviruses of interest, using genetic and serologic assays. Of the 255 CSF samples analyzed, 3.53% (09/255) were positive for arboviruses presenting mainly as meningitis, encephalitis, and cerebrovascular events, of which ZIKV was detected in 2.74% (7/255), DENV in 0.78% (2/255), in addition to an identified ILHV infection that was described previously. All the cases were detected in adults aged 18 to 74 years old. Our findings highlight the scientific and clinical importance of neurological syndromes associated with arboviruses and demonstrate the relevance of specific laboratory methods to achieve accurate diagnoses as well as highlight the true dimension of these diseases to ultimately improve public health planning and medical case management.
虫媒病毒感染是越来越重要的疾病病因，其神经表现谱尚不完全清楚。本研究旨在回顾性评估神经症状患者的脑脊液样本中虫媒病毒的发生率，以辅助中枢和周围神经系统疾病的诊断。我们使用遗传和血清学检测方法，对 2016 年 1 月至 2017 年 12 月期间收集的 255 份脑脊液样本进行了登革热病毒 (DENV1-4)、寨卡病毒 (ZIKV) 和基孔肯雅热病毒 (CHIKV) 以及其他有神经嗜性的虫媒病毒的检测。在分析的 255 份脑脊液样本中，有 3.53%（09/255）呈虫媒病毒阳性，主要表现为脑膜炎、脑炎和脑血管事件，其中 2.74%（7/255）检测到寨卡病毒，0.78%（2/255）检测到登革热病毒，此外还发现了以前描述过的 ILHV 感染。所有病例均发生在 18 至 74 岁的成年人中。我们的研究结果强调了与虫媒病毒相关的神经综合征的科学和临床重要性，并证明了特定实验室方法在实现准确诊断方面的相关性，同时也突出了这些疾病的真实规模，最终可改善公共卫生规划和医疗病例管理。
