Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
Lower Saxony Center for Biomedical Engineering, Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, Germany.
Int J Mol Sci. 2022 Jul 22;23(15):8058. doi: 10.3390/ijms23158058.
Microbial infection and insufficient tissue formation are considered to be the two main causes of dental implant failure. Novel studies have focused on designing dual-functional strategies to promote antibacterial properties and improve tissue cell response simultaneously. In this study, we investigated the antibacterial properties and cytocompatibility of silver nitrate (AgNO) and strontium acetate (SrAc) in a mono-culture setup for dental application. Additionally, we defined the therapeutic window between the minimum inhibitory concentration against pathogenic bacteria and maximum cytocompatible dose in the case of combined applications in a co-culture setup. Antibacterial properties were screened using and cell response experiments were performed with osteoblastic cells (MC3T3) and fibroblastic cells (NIH3T3). The osteoinductive behavior was investigated separately on MC3T3 cells using alizarin red staining. A therapeutic window for AgNO as well as SrAc applications could be defined in the case of MC3T3 cells while the cytocompatibility of NIH3T3 cells was compromised for all concentrations with an antibacterial effect. However, the combined application of AgNO/SrAc caused an enhanced antibacterial effect and opened a therapeutic window for both cell lines. Enhanced mineralization rates could be observed in cultures containing SrAc. In conclusion, we were able to demonstrate that adding SrAc to AgNO not only intensifies antibacterial properties but also exhibits bone inductive characteristics, thereby offering a promising strategy to combat peri-implantitis and at the same time improve osseointegration in implant therapy.
微生物感染和组织形成不足被认为是导致种植牙失败的两个主要原因。新的研究集中于设计双功能策略，以同时促进抗菌性能和改善组织细胞反应。在这项研究中，我们研究了硝酸银（AgNO）和醋酸锶（SrAc）在用于牙科的单培养物设置中的抗菌性能和细胞相容性。此外，我们在共培养物设置中定义了针对组合应用的最小抑菌浓度（MIC）与最大细胞相容性剂量之间的治疗窗口。使用 进行了抗菌性能筛选，并用成骨细胞（MC3T3）和成纤维细胞（NIH3T3）进行了细胞反应实验。用茜素红染色分别在 MC3T3 细胞上研究了成骨诱导行为。可以在 MC3T3 细胞的情况下为 AgNO 和 SrAc 的应用定义治疗窗口，而 NIH3T3 细胞的细胞相容性在具有抗菌作用的所有浓度下都受到损害。然而，AgNO/SrAc 的联合应用导致了增强的抗菌效果，并为两种细胞系开辟了治疗窗口。含有 SrAc 的培养物中可以观察到增强的矿化速率。总之，我们能够证明向 AgNO 中添加 SrAc 不仅增强了抗菌性能，而且还表现出骨诱导特性，从而提供了一种有前途的策略来对抗种植体周围炎，同时改善种植体治疗中的骨整合。
