Polymer Biomaterial Design and Synthesis Laboratory, Department of Chemistry, Yogi Vemana University, Kadapa, India.
Department of Chemistry, Sri Krishnadeveraya University, Anantapuramu, India.
Drug Deliv. 2022 Dec;29(1):2481-2490. doi: 10.1080/10717544.2022.2100512.
The development of effective carriers enabling combination cancer therapy is of practical importance due to its potential to enhance the effectiveness of cancer treatment. However, most of the reported carriers are monofunctional in nature. The carriers that can be applied to concomitantly mediate multiple treatment modalities are highly deficient. This study fills this gap by reporting the design and fabrication of ROS-generating carbohydrate-based pH-responsive beads with intrinsic anticancer therapy and multidrug co-delivery capacity for combination cancer therapy. Sodium alginate (SA) microspheres and reduced graphene oxide (rGO)-embedded chitosan (CS) beads are developed via emulsion-templated ionic gelation for a combination therapy involving co-delivery of curcumin (CUR) and 5-fluororacil (5-FU). Drug-encapsulated microbeads are characterized by FTIR, DSC, TGA, XRD, and SEM. 5-FU and CUR-encapsulated microbeads are subjected to drug release studies at pH 6.8 and 1.2 at 37 °C. Various release kinetic parameters are evaluated. The results show that the Korsmeyer-Peppas model and non-Fickian release kinetics are best suited. The microspheres and microbeads are found to effectively act against MCF7 cells and show intrinsic anticancer capacity. These results indicate the promising performance of our beads in mediating combination drug therapy to improve the effectiveness of cancer treatment.
由于能够增强癌症治疗效果的潜力，开发能够实现联合癌症治疗的有效载体具有实际意义。然而，大多数报道的载体在性质上都是单功能的。能够同时介导多种治疗方式的载体非常缺乏。本研究通过报告设计和制造具有内在抗癌治疗和多药共递送能力的用于联合癌症治疗的产生活性氧（ROS）的基于碳水化合物的 pH 响应珠来填补这一空白。通过乳液模板化离子凝胶化制备海藻酸钠（SA）微球和嵌入还原氧化石墨烯（rGO）的壳聚糖（CS）珠，用于联合治疗，共递送姜黄素（CUR）和 5-氟尿嘧啶（5-FU）。通过傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）、热重分析（TGA）、X 射线衍射（XRD）和扫描电子显微镜（SEM）对载药微球进行了表征。在 37°C 下，在 pH 6.8 和 1.2 下对载有 5-FU 和 CUR 的微球进行了药物释放研究。评估了各种释放动力学参数。结果表明，Korsmeyer-Peppas 模型和非 Fickian 释放动力学最适合。发现微球和微珠能够有效地对抗 MCF7 细胞并显示出内在的抗癌能力。这些结果表明我们的珠在介导联合药物治疗以提高癌症治疗效果方面具有广阔的应用前景。
