恒河猴与大鼠皮质脊髓投射组的分化。
Rhesus macaque versus rat divergence in the corticospinal projectome.
机构信息
Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA.
Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA; Veterans Administration Medical Center, La Jolla, CA, USA.
出版信息
Neuron. 2022 Sep 21;110(18):2970-2983.e4. doi: 10.1016/j.neuron.2022.07.002. Epub 2022 Aug 1.
We used viral intersectional tools to map the entire projectome of corticospinal neurons associated with fine distal forelimb control in Fischer 344 rats and rhesus macaques. In rats, we found an extraordinarily diverse set of collateral projections from corticospinal neurons to 23 different brain and spinal regions. Remarkably, the vast weighting of this "motor" projection was to sensory systems in both the brain and spinal cord, confirmed by optogenetic and transsynaptic viral intersectional tools. In contrast, rhesus macaques exhibited far heavier and narrower weighting of corticospinal outputs toward spinal and brainstem motor systems. Thus, corticospinal systems in macaques primarily constitute a final output system for fine motor control, whereas this projection in rats exerts a multi-modal integrative role that accesses far broader CNS regions. Unique structural-functional correlations can be achieved by mapping and quantifying a single neuronal system's total axonal output and its relative weighting across CNS targets.
我们使用病毒交叉工具来绘制与费舍尔 344 大鼠和恒河猴精细远端前肢控制相关的皮质脊髓神经元的整个投射组。在大鼠中，我们发现了来自皮质脊髓神经元到 23 个不同脑和脊髓区域的非常多样化的侧支投射。值得注意的是，这种“运动”投射的巨大权重是大脑和脊髓中的感觉系统，这一点得到了光遗传学和跨突触病毒交叉工具的证实。相比之下，恒河猴的皮质脊髓输出对脊髓和脑干运动系统的权重要大得多且狭窄得多。因此，猕猴的皮质脊髓系统主要构成精细运动控制的最终输出系统，而大鼠中的这种投射则发挥了多模态整合作用，可访问更广泛的中枢神经系统区域。通过绘制和量化单个神经元系统的总轴突输出及其在中枢神经系统目标中的相对权重，可以实现独特的结构-功能相关性。
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