Shaheen Ghazala, Ashfaq Arslan, Khawar Anum, Jamil Qurratul Ain, Parveen Rashida, Hadi Faheem, Ghauri Aymen Owais, Shirazi Jafir Hussain, Asif Hafiz Muhammad, Shamim Tahira, Sumreen Laila, Ali Tanveer, Akram Muhammad, Noor Raessa, Mehmood Amna, Sajid Farhan
Department of Eastern Medicine, University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences. The Islamia University of Bahawalpur, Pakistan.
Faculty of Eastern Medicine and Natural Sciences, Ziauddin University Karachi, Pakistan.
Pharm Pract (Granada). 2022 Apr-Jun;20(2):2647. doi: 10.18549/PharmPract.2022.2.2647. Epub 2022 Apr 26.
Aim of present study was to assess pharmacological (antioxidant, antibacterial & antifungal) potential of Operculina terpathum seeds. Ethanolic extract was prepared and its phytochemical evaluation show the different chemical compounds such as carbohydrates, phenols, tannin, flavonoids, cardiac glycosides, steroids, alkaloids and proteins. FTIR spectra showed the presence of organic acids, hydroxyl and phenolic compounds, amino groups, aliphatic compounds, functional groups such as amide, ketone, aldehyde, aromatics and halogen compounds. Antioxidant activity of the Operculina terpathum alcoholic extract was performed by DPPH method and it showed 97.13%whereas IC50±SEM (µg/ml) was 1.425±0.16. Antibacterial activity was performed against different bacterial strains and results were comparable with that of standard. Maximum antibacterial activity was exhibited by Bacillus subtillis (28.33±2 mm) and Bacillus pumilus (25.33±2 mm) respectively. Antifungal activity was also performed and it showed maximum activity against Aspergillus flavous and Candida albicans6±1, 5±1mm respectively. These results showed that Operculina terpathum has good antibacterial and antifungal activity against different microbes and it could be used as an alternative to antibiotics, as the antibiotics resistance is very common now a days.
本研究的目的是评估盒果藤种子的药理（抗氧化、抗菌和抗真菌）潜力。制备了乙醇提取物，其植物化学评估显示出不同的化合物，如碳水化合物、酚类、单宁、黄酮类、强心苷、甾体、生物碱和蛋白质。傅里叶变换红外光谱显示存在有机酸、羟基和酚类化合物、氨基、脂肪族化合物、酰胺、酮、醛、芳烃和卤素化合物等官能团。采用DPPH法测定盒果藤乙醇提取物的抗氧化活性，其抗氧化率为97.13%，而IC50±SEM（μg/ml）为1.425±0.16。对不同细菌菌株进行了抗菌活性测试，结果与标准品相当。枯草芽孢杆菌（28.33±2 mm）和短小芽孢杆菌（25.33±2 mm）分别表现出最大的抗菌活性。还进行了抗真菌活性测试，结果显示对黄曲霉和白色念珠菌的活性最大，分别为6±1、5±1mm。这些结果表明，盒果藤对不同微生物具有良好的抗菌和抗真菌活性，由于现在抗生素耐药性非常普遍，它可以用作抗生素的替代品。
