骨关节炎滑液中蛋白聚糖的分光光度法测量
Spectrophotometric measurement of proteoglycans in osteoarthritic synovial fluid.
作者信息
Carroll G J
出版信息
Ann Rheum Dis. 1987 May;46(5):375-9. doi: 10.1136/ard.46.5.375.
Abstract
Cartilage glycosaminoglycans (GAGs) were measured by a spectrophotometric assay in synovial fluid obtained from 30 normal bovine hock joints and 15 osteoarthritic human knee joints. Results were compared with those obtained by radioimmunoassay (RIA). The spectrophotometric method (dimethylmethylene blue (DMB) assay) was found to be simple, safe, and sufficiently reproducible to be of potential value for serial measurement of sulphated GAGs in arthritic joint fluids. The nature of the proteoglycans present in normal bovine and osteoarthritic human synovial fluid was examined by gel chromatography. Whereas normal bovine synovial fluid contained only small molecular weight proteoglycans, osteoarthritic human synovial fluid contained aggregated proteoglycans and predominantly high molecular weight proteoglycan subunits.
摘要
通过分光光度法对从30个正常牛跗关节和15个骨关节炎患者膝关节获取的滑液中的软骨糖胺聚糖（GAGs）进行了测量。将结果与通过放射免疫测定法（RIA）获得的结果进行了比较。发现分光光度法（二甲基亚甲基蓝（DMB）测定法）简单、安全且具有足够的可重复性，对于关节炎关节液中硫酸化GAGs的系列测量具有潜在价值。通过凝胶色谱法研究了正常牛和骨关节炎患者滑液中蛋白聚糖的性质。正常牛滑液仅含有小分子量的蛋白聚糖，而骨关节炎患者滑液含有聚集的蛋白聚糖且主要是高分子量的蛋白聚糖亚基。
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