School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210046, China.
Environmental Sciences Department, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia.
Appl Microbiol Biotechnol. 2022 Sep;106(17):5385-5397. doi: 10.1007/s00253-022-12101-y. Epub 2022 Aug 5.
As important chemical raw materials and potential nutritional supplements, microbial lipids play an important role in ensuring economic development, food security, and energy security. Compared with non-natural hosts, oleaginous yeasts exhibit obvious advantages in lipid yield and productivity and have great potential to be genetically engineered into an oil cell factory. The main bottleneck in the current oleaginous yeasts engineering is the lack of genetic manipulation tools. Fortunately, the rapid development of synthetic biology has provided numerous new approaches, resources, and ideas for the field. Most importantly, gene editing technology mediated by CRISPR/Cas systems has been successfully applied to some oleaginous yeasts, almost completely rewriting the development pattern of genetic manipulation technology applicable. This paper reviews recent progress in genetic technology with regard to oleaginous yeasts, with a special focus on transformation methods and genome editing tools, discussing the effects of some important genetic parts. KEY POINTS: •Contribution of microbiotechnology in food safety and biofuel by oleaginous yeasts. •Advancement of genetic manipulation and transformation for oleaginous yeasts.
作为重要的化学原料和潜在的营养补充剂，微生物油脂在确保经济发展、粮食安全和能源安全方面发挥着重要作用。与非天然宿主相比，产油酵母在产油量和生产力方面表现出明显的优势，具有很大的潜力被遗传工程改造为油脂细胞工厂。目前产油酵母工程的主要瓶颈是缺乏遗传操作工具。幸运的是，合成生物学的快速发展为该领域提供了许多新的方法、资源和思路。最重要的是，CRISPR/Cas 系统介导的基因编辑技术已成功应用于一些产油酵母，几乎完全改写了可应用遗传操作技术的发展模式。本文综述了近年来产油酵母遗传技术的最新进展，特别关注转化方法和基因组编辑工具，讨论了一些重要遗传元件的作用。要点：• 产油酵母在食品安全和生物燃料方面的微生物技术贡献。• 产油酵母遗传操作和转化的进展。
