The Second Clinical Medical College, Lanzhou University, Lanzhou, 730030, Gansu, China.
Department of Orthopedics, Lanzhou University Second Hospital, No. 82 Cuiyingmen Street, Lanzhou, 730030, Gansu, China.
Anal Bioanal Chem. 2022 Oct;414(24):7123-7141. doi: 10.1007/s00216-022-04253-7. Epub 2022 Aug 13.
Exosomes are extracellular vesicles with a diameter ranging from 30 to 150 nm, which are an important medium for intercellular communication and are closely related to the progression of certain diseases. Therefore, exosomes are considered promising biomarkers for the diagnosis of specific diseases, and thereby, treatments based on exosomes are being widely examined. For exosome-related research, a rapid, simple, high-purity, and recovery isolation method is the primary prerequisite for exosomal large-scale application in medical practice. Although there are no standardized methods for exosome separation and analysis, various techniques have been established to explore their biochemical and physicochemical properties. In this review, we analyzed the progress in exosomal isolation strategies and proposed our views on the development prospects of various exosomal isolation techniques.
外泌体是一种细胞外囊泡，直径在 30 到 150nm 之间，是细胞间通讯的重要介质，与某些疾病的进展密切相关。因此，外泌体被认为是特定疾病诊断的有前途的生物标志物，基于外泌体的治疗方法正在被广泛研究。对于外泌体相关的研究，快速、简单、高纯度和高回收率的分离方法是外泌体在医学实践中大规模应用的首要前提。尽管外泌体的分离和分析方法尚未标准化，但已经建立了各种技术来探索它们的生化和物理化学特性。在这篇综述中，我们分析了外泌体分离策略的进展，并对外泌体分离技术的发展前景提出了我们的看法。
