Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmaceutics, Faculty of Pharmacy, Mashhad University of Medical Science, Mashhad, Iran.
J Tissue Viability. 2022 Nov;31(4):649-656. doi: 10.1016/j.jtv.2022.07.009. Epub 2022 Jul 31.
Chitosan-based hydrogels as wound dressings are expected to improve the efficiency of the wound-healing process. Fabrication of the composite structure of Aloe vera and biopolymeric hydrogels has been demonstrated to promote the wound-healing process through protection against a wide spectrum of microbes, and enhanced cell adhesion and differentiation. Therefore, the present study investigates to development of improved CHO/Aloe hydrogel for improving the wound-healing process in an animal model.
CHO hydrogel with Aloe was prepared, and its properties were evaluated in terms of viscosity, antibacterial activity, and ints In-vivo wound-healing efficiency in full-thickness wounds of rat models. Physical examination of wound-healing efficiency of CHO/Aleo hydrogel was evaluated by analyzing total wound closure, recovery percentage, and the epiderm thickness of wounds. Histological evaluation was performed using hematoxylin and eosin staining to evaluate the re-epithelialization, inflammatory response, granulation tissue formation, and fibrotic tissue formation.
The results showed a significantly higher wound-healing rate of the CHO/Aleo group compared to other groups at 3,7,14 days (p < 0.05). After 14 days of treatment, the best healing effect was observed in the CHO/Aleo gel with the highest tissue tension compared with other groups (p < 0.05). Histological findings indicated a significant difference in inflammatory response between control and treatment groups after three days of treatment (p < 0.05). Epidermal thickness was also significantly thicker in the CHO/Aleo gel group than others (p < 0.05).
The present study an improved the effective topical drug-delivery system by CHO/Aloe hydrogel with the potential to reduce inflammation over time, allowing the body to recover more quickly and better re-epithelialization for improving the wound-healing procedures.
壳聚糖基水凝胶作为伤口敷料有望提高伤口愈合过程的效率。已经证明，通过保护免受广谱微生物的侵害以及增强细胞黏附和分化，制备芦荟和生物聚合物水凝胶的复合结构可以促进伤口愈合过程。因此，本研究旨在开发改进的 CHO/芦荟水凝胶，以改善动物模型中的伤口愈合过程。
制备了 CHO 水凝胶与芦荟的混合物，并从黏度、抗菌活性和体内方面评价其性能在大鼠全层伤口模型中的伤口愈合效率。通过分析总伤口闭合、恢复百分比和伤口表皮厚度来评估 CHO/芦荟水凝胶的伤口愈合效率的体格检查。使用苏木精和伊红染色进行组织学评估，以评估再上皮化、炎症反应、肉芽组织形成和纤维组织形成。
结果表明，CHO/芦荟组在第 3、7、14 天时的伤口愈合率明显高于其他组（p<0.05）。治疗 14 天后，与其他组相比，CHO/Aloe 凝胶的组织张力最高，观察到最佳的愈合效果（p<0.05）。组织学发现，治疗 3 天后，对照组和治疗组之间的炎症反应有显著差异（p<0.05）。CHO/Aloe 凝胶组的表皮厚度也明显比其他组厚（p<0.05）。
本研究通过 CHO/Aloe 水凝胶改善了有效的局部药物递送系统，具有随着时间的推移减轻炎症的潜力，使身体更快地恢复并更好地再上皮化，从而改善伤口愈合过程。
