小鼠补体的灵敏溶血测定法。
A sensitive haemolytic assay of mouse complement.
作者信息
Berden J H, Hagemann J F, Koene R A
出版信息
J Immunol Methods. 1978;23(1-2):149-59. doi: 10.1016/0022-1759(78)90118-7.
PMID:359711
Abstract
Under appropriate conditions the haemolytic activity of mouse complement can be measured in a 51Cr release assay. Modifications that increase the sensitivity are the following: (a) mouse 7 S anti-SRBC antibodies as amboceptor; (b) low amount of target cells; (c) ionic strength of 0.13 M NaCl in the test medium; (d) incubation temperature of 30 degrees C; (e) incubation time of 90 min; (f) total reaction volume of 1 ml.
摘要
在适当条件下，小鼠补体的溶血活性可通过51Cr释放试验来测定。提高灵敏度的改良方法如下：(a) 用小鼠7S抗绵羊红细胞抗体作为双价抗体；(b) 使用少量靶细胞；(c) 试验介质中NaCl的离子强度为0.13M；(d) 孵育温度为30℃；(e) 孵育时间为90分钟；(f) 总反应体积为1毫升。
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