Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, PR China.
Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, PR China.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005484.
Strain 155047 was recovered from human sputum in China in 2021. Preliminary species identification based on limited phenotypic tests assigned the strain to the genus of the family . The genome sequence of the strain was obtained and had ≤84.43 % average nucleotide identity (ANI) and ≤26.3 % DNA-DNA hybridization (isDDH) values with the genomes of type strains of known species. The highest ANI and isDDH matches were with and , respectively. The ANI and isDDH values support that the strain belongs to a novel species of the family . Phylogenomic analysis based on core genes revealed that strain 155047 was located in the lineage. The highest ANI and average amino acid identity values between 155047 and any species of the lineage were 84.43 % and 90.21 %, respectively, lower than the maximum inter-genus pairwise values. This indicates that 155047 belongs to a novel species of a novel genus in the lineage. Strain 155047 could be differentiated from , , and species by a negative reaction for β-galactosidase and the ability to produce acid from l-fucose but not from sucrose. The names gen. nov. and sp. nov. are proposed for the novel genus and species, respectively. The type strain is 155047 (= GDMCC 1.2980=JCM 35262).
155047 株于 2021 年从中国的人痰中分离得到。基于有限的表型试验的初步种鉴定将该菌株分配到 科的属。该菌株的基因组序列已获得,其与已知种的模式菌株的基因组的平均核苷酸同一性(ANI)值≤84.43%,DNA-DNA 杂交(isDDH)值≤26.3%。ANI 和 isDDH 的最高匹配分别为 和 。ANI 和 isDDH 值支持该菌株属于科的一个新种。基于核心基因的系统发育分析表明,菌株 155047 位于 进化枝。155047 与任何 进化枝物种之间的最高 ANI 和平均氨基酸同一性值分别为 84.43%和 90.21%,均低于最大种间成对值。这表明 155047 属于该进化枝中新属和新种的一个菌株。155047 株可通过β-半乳糖苷酶反应阴性和从 l-岩藻糖产酸但不从蔗糖产酸来与 、 、 和 种相区分。提议用新属和新种的名称 gen. nov. 和 sp. nov. 分别命名新属和新种。模式菌株为 155047(=GDMCC 1.2980=JCM 35262)。