Vascular Biology and Molecular Pathology, Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Oral Diagnosis and Medicine, Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Sci Rep. 2022 Aug 18;12(1):14050. doi: 10.1038/s41598-022-18367-6.
Cetylpyridinium chloride (CPC), a quaternary ammonium compound, which is present in mouthwash, is effective against bacteria, fungi, and enveloped viruses. This study was conducted to explore the antiviral effect of CPC on SARS-CoV-2. There are few reports on the effect of CPC against wild-type SARS-CoV-2 at low concentrations such as 0.001%-0.005% (10-50 µg/mL). Interestingly, we found that low concentrations of CPC suppressed the infectivity of human isolated SARS-CoV-2 strains (Wuhan, Alpha, Beta, and Gamma) even in saliva. Furthermore, we demonstrated that CPC shows anti-SARS-CoV-2 effects without disrupting the virus envelope, using sucrose density analysis and electron microscopic examination. In conclusion, this study provided experimental evidence that CPC may inhibit SARS-CoV-2 infection even at lower concentrations.
氯化十六烷基吡啶（CPC）是一种季铵化合物，存在于漱口水中，对细菌、真菌和包膜病毒有效。本研究旨在探索 CPC 对 SARS-CoV-2 的抗病毒作用。目前，关于 CPC 在低浓度（如 0.001%-0.005%，即 10-50μg/mL）下对野生型 SARS-CoV-2 的作用的报道较少。有趣的是，我们发现低浓度的 CPC 抑制了人分离的 SARS-CoV-2 株（武汉株、Alpha、Beta 和 Gamma）的感染力，即使在唾液中也是如此。此外，我们还通过蔗糖密度分析和电子显微镜检查证明，CPC 具有抗 SARS-CoV-2 作用，而不会破坏病毒包膜。总之，本研究提供了实验证据，表明 CPC 即使在较低浓度下也可能抑制 SARS-CoV-2 的感染。
