Goller Carlos C, Johnson Graham T, Casimo Kaitlyn
Department of Biological Sciences, North Carolina State University.
Biotechnology Program, North Carolina State University.
CourseSource. 2022;9. doi: 10.24918/cs.2022.3. Epub 2022 Jan 27.
Organelle structure has been studied and visualized for decades; however, publicly available databases that use improved high-throughput microscopy of gene-edited cell lines have recently revolutionized the amount and quality of information now available for use in undergraduate classes. This lesson demonstrates how the use of high-throughput (HT) microscopy has generated data describing organelle structure and variability. Students access, analyze, and evaluate cell structure images using the Allen Institute for Cell Science's Allen Cell Explorer. Students synthesize the information to make recommendations and propose a future experiment. Using web-based tools and a realistic scenario that merges antimicrobial drug screens with eukaryotic cell perturbations and structure, this case study provides a guided tour of the powerful applications of high-throughput microscopy.
细胞器结构已被研究并可视化了数十年；然而，最近出现的公开可用数据库利用经过改进的基因编辑细胞系高通量显微镜技术，彻底改变了目前可供本科课程使用的信息数量和质量。本课程展示了高通量（HT）显微镜技术如何生成描述细胞器结构和变异性的数据。学生使用艾伦细胞科学研究所的艾伦细胞浏览器访问、分析和评估细胞结构图像。学生综合这些信息以提出建议并设计未来的实验。通过基于网络的工具以及将抗菌药物筛选与真核细胞扰动和结构相结合的现实场景，本案例研究对高通量显微镜技术的强大应用进行了一次引导式探索。
