Key Laboratory of Marine Drugs, the Ministry of Education of China, School of Medicine and Pharmacy, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
College of Pharmaceutical Sciences, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnostics of Education Ministry of China, Hebei University, Baoding 071002, China.
Mar Drugs. 2022 Jul 28;20(8):486. doi: 10.3390/md20080486.
Three new polyketides, eutyketides A and B ( and ) and cytosporin X (), along with four known compounds (-), were obtained from the marine-derived fungus . The planar structures of and were elucidated by extensive HRMS and 1D and 2D NMR analyses. Their relative configurations of C-13 and C-14 were determined with chemical conversions by introducing an acetonylidene group. The absolute configurations of - were determined by comparing their experimental electronic circular dichroism (ECD) data with their computed ECD results. All of the isolated compounds were tested for their anti-inflammatory activities on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages. Compounds and showed stronger anti-inflammatory activities than the other compounds, with the inhibition of 49.0% and 54.9% at a concentration of 50.0 µg/mL, respectively.
从海洋来源的真菌中获得了三种新的聚酮化合物，即 eutyketides A 和 B（和）和 cytosporin X（），以及四种已知化合物（-）。通过 HRMS 和 1D 和 2D NMR 分析，阐明了和的平面结构。通过引入乙酰基来进行化学转化，确定了 C-13 和 C-14 的相对构型。通过比较实验电子圆二色谱（ECD）数据与计算 ECD 结果，确定了-的绝对构型。所有分离得到的化合物均在 LPS 诱导的 RAW 264.7 巨噬细胞中一氧化氮产生的抗炎活性方面进行了测试。化合物和表现出比其他化合物更强的抗炎活性，在 50.0 µg/mL 的浓度下，抑制率分别为 49.0%和 54.9%。
