Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Provincial Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), Guangdong Institute of Microbiology, South China, Guangzhou 510070, P R China.
Nat Prod Res. 2013;27(14):1298-304. doi: 10.1080/14786419.2012.733393. Epub 2012 Oct 12.
Two new polyketides, 7,8-dihydroxy-3,5,7-trimethyl-8,8a-dihydro-1H-isochromen-6(7H)-one (1) and 6-(hydroxymethyl)-2,2-dimethyl-3,4-dihydro-2H-chromene-3,4-diol (2), together with a known nitrogen-containing polyketide (cytochalasin-type of metabolites), [12]-cytochalasin (3), have been isolated from the fermentation broth of a marine sediment-derived fungus Eutypella scoparia FS26 obtained from the South China Sea. Their structures were elucidated by spectroscopic methods, mainly 1D and 2D NMR spectroscopic techniques. The absolute configurations of compound 1 were determined by NOESY analysis and the literature data were compared with circular dichroism (CD) spectroscopy. The cytotoxic effects on MCF-7, NCI-H460 and SF-268 cell lines of all compounds were evaluated by the sulforhodamine B method.
从南海来源的海洋沉积物真菌 Eutypella scoparia FS26 的发酵液中分离得到了两个新的聚酮化合物，7,8-二羟基-3,5,7-三甲基-8,8a-二氢-1H-异色满-6(7H)-酮（1）和 6-（羟甲基）-2,2-二甲基-3,4-二氢-2H-色满-3,4-二醇（2），以及一个已知的含氮聚酮化合物（细胞松弛素型代谢物），[12]-细胞松弛素（3）。通过光谱方法，主要是 1D 和 2D NMR 光谱技术，阐明了它们的结构。通过 NOESY 分析确定了化合物 1 的绝对构型，并与文献数据进行了比较，采用圆二色（CD）光谱法。通过磺基罗丹明 B 法评估了所有化合物对 MCF-7、NCI-H460 和 SF-268 细胞系的细胞毒性作用。
