Waniczek Dariusz, Świętochowska Elżbieta, Śnietura Mirosław, Kiczmer Paweł, Lorenc Zbigniew, Muc-Wierzgoń Małgorzata
Department of Surgical Nursing and Propaedeutics of Surgery, Faculty of Health Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, Poland.
Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Metabolites. 2022 Jul 28;12(8):704. doi: 10.3390/metabo12080704.
Colorectal cancer is one of the most prevalent cancers worldwide. There is a great interest and need to find simple, inexpensive, and minimally invasive diagnostic tests. The aim of the study was to analyze the salivary concentrations of chemerin, α-defensin 1, and TNF-α in colorectal cancer (CRC) patients and in a healthy control group. The concentration of these proteins was simultaneously determined in the serum of subjects. We also aimed to assess the correlation of these results and selected clinicopathological features. This prospective study was comprised of 39 CRC patients and 40 control group patients. Salivary and serum concentrations were determined by enzyme immunoassays. The salivary and serum concentrations of chemerin, α-defensin 1, and TNF-α were significantly higher in cancer patients compared to the control group. No correlation was found between concentrations of the proteins and the clinical stage of cancer and tumor location. The ROC curve analysis showed that although salivary concentrations of all proteins showed 100% sensitivity and 100% specificity, serum concentrations of the analyzed proteins were characterized by 100% sensitivity and over 90% specificity. The assessment of chemerin, α-defensin 1, and TNF-α concentrations in saliva seem to have great potential as quick and useful biomarkers in the early diagnosis of CRC.
结直肠癌是全球最常见的癌症之一。人们对寻找简单、廉价且微创的诊断测试有着浓厚的兴趣和需求。本研究的目的是分析结直肠癌（CRC）患者和健康对照组中chemerin、α-防御素1和TNF-α的唾液浓度。同时测定了受试者血清中这些蛋白质的浓度。我们还旨在评估这些结果与选定的临床病理特征之间的相关性。这项前瞻性研究包括39例CRC患者和40例对照组患者。通过酶免疫测定法测定唾液和血清浓度。与对照组相比，癌症患者唾液和血清中chemerin、α-防御素1和TNF-α的浓度显著更高。未发现蛋白质浓度与癌症临床分期和肿瘤位置之间存在相关性。ROC曲线分析表明，尽管所有蛋白质的唾液浓度显示出100%的敏感性和100%的特异性，但所分析蛋白质的血清浓度具有100%的敏感性和超过90%的特异性。评估唾液中chemerin、α-防御素1和TNF-α的浓度似乎具有作为CRC早期诊断中快速且有用的生物标志物的巨大潜力。
