Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania Luigi Vanvitelli, Via Vivaldi 43, 81100 Caserta, Italy.
Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, E-47011 Valladolid, Spain.
Toxins (Basel). 2022 Aug 19;14(8):566. doi: 10.3390/toxins14080566.
Ribosome-inactivating proteins (RIPs) are known as RNA N-glycosylases. They depurinate the major rRNA, damaging ribosomes and inhibiting protein synthesis. Here, new single-chain (type-1) RIPs named sodins were isolated from the seeds (five proteins), edible leaves (one protein) and roots (one protein) of L. Sodins are able to release Endo's fragment when incubated with rabbit and yeast ribosomes and inhibit protein synthesis in cell-free systems (IC = 4.83-79.31 pM). In addition, sodin 5, the major form isolated from seeds, as well as sodin eL and sodin R, isolated from edible leaves and roots, respectively, display polynucleotide:adenosine glycosylase activity and are cytotoxic towards the Hela and COLO 320 cell lines (IC = 0.41-1200 nM), inducing apoptosis. The further characterization of sodin 5 reveals that this enzyme shows a secondary structure similar to other type-1 RIPs and a higher melting temperature (Tm = 76.03 ± 0.30 °C) and is non-glycosylated, as other sodins are. Finally, we proved that sodin 5 possesses antifungal activity against .
核糖体失活蛋白（RIPs）被称为 RNA N-糖基化酶。它们使主要 rRNA 脱嘌呤，破坏核糖体并抑制蛋白质合成。在这里，从 L. 的种子（五种蛋白）、可食用的叶子（一种蛋白）和根（一种蛋白）中分离出新型的单链（I 型）RIPs，命名为 sodins。当与兔和酵母核糖体孵育时，sodins 能够释放 Endo 的片段并抑制无细胞体系中的蛋白质合成（IC = 4.83-79.31 pM）。此外，从种子中分离出的主要形式的 sodin 5，以及从可食用的叶子和根中分离出的 sodin eL 和 sodin R，均显示多核苷酸：腺嘌呤糖苷酶活性，并对 Hela 和 COLO 320 细胞系具有细胞毒性（IC = 0.41-1200 nM），诱导细胞凋亡。对 sodin 5 的进一步表征表明，该酶显示出与其他 I 型 RIP 相似的二级结构，并且具有更高的熔点（Tm = 76.03 ± 0.30°C），并且像其他 sodins 一样是非糖基化的。最后，我们证明 sodin 5 对 具有抗真菌活性。
