采用离子液体双水相体系高效分离蛋白质和多糖。
Efficient Separation of Proteins and Polysaccharides from Using Aqueous Two-Phase System with Ionic Liquids.
机构信息
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
Laboratory of Quality Assessment, Shenyang Institute of Food and Drug Control, Shenyang 110122, China.
出版信息
Molecules. 2022 Aug 18;27(16):5284. doi: 10.3390/molecules27165284.
By applying the hydrophilic ionic liquid, 1-butyl-3-methylimidazolium chloride ([Cmim]Cl), and inorganic salts (KPO), an ionic liquid aqueous two-phase system (ILATPS) was established for the separation of polysaccharides (DhPs) and proteins. The effects of inorganic salt concentration, IL quantity, crude DhPs concentration, pH value and temperature were studied to achieve the optimal condition. With the best combination of ILATPS (1.75 g KPO, 1.25 g [Cmim]Cl, 10 mg crude DhPs and 5.0 mL ddHO at pH 7.0 under 25 °C), the extraction efficiency rates for DhPs and proteins were 93.4% and 90.2%, respectively. The processed DhPs retrieved from the lower salt-rich phase comprised mannose, glucose, galactose, arabinose, and galacturonic acid with a molar ratio of 185:71:1.5:1:1 and the molecular weight was 2.14 × 10 Da. This approach is fast, simple and environmentally friendly. It provides a new insight into purifying functional polysaccharides of plant origin.
通过应用亲水性离子液体 1-丁基-3-甲基咪唑氯化物([Cmim]Cl)和无机盐(KPO)，建立了离子液体双水相体系(ILATPS)，用于分离多糖(DhPs)和蛋白质。研究了无机盐浓度、IL 用量、粗 DhPs 浓度、pH 值和温度的影响，以达到最佳条件。在最佳 ILATPS 组合下(1.75 g KPO、1.25 g [Cmim]Cl、10 mg 粗 DhPs 和 5.0 mL ddHO，在 25°C 下 pH 为 7.0)，DhPs 和蛋白质的提取效率分别为 93.4%和 90.2%。从富含低盐的下相中回收的处理后的 DhPs 由甘露糖、葡萄糖、半乳糖、阿拉伯糖和半乳糖醛酸组成，摩尔比为 185:71:1.5:1:1，分子量为 2.14×10 Da。该方法快速、简单、环保，为植物来源的功能性多糖的纯化提供了新的思路。
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