State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academic of Sciences, Wuhan 430072, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; Xianning Engineering Research Center for Healthy Environment, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, PR China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academic of Sciences, Wuhan 430072, PR China.
Sci Total Environ. 2022 Dec 10;851(Pt 2):158260. doi: 10.1016/j.scitotenv.2022.158260. Epub 2022 Aug 27.
Glyphosate (GLY) contamination widely occurred in aquatic environments including aquaculture systems and raised hazard to aquatic organisms such as fish. Probiotics have been reported to alleviate contaminants-induced toxicity. However, whether probiotics could reduce the health risk of GLY to fish remain unknown. Here we investigated the impacts of GLY on crucian carp (Carassius auratus) by focusing on the protective roles of two commonly used aquaculture probiotics, Bacillus coagulans (BC) and Clostridium butyricum (CB). Exposure to GLY significantly caused growth retardation and reduced visceral fat and intestinal lipase activity in crucian carp. 16S rRNA sequencing indicated that dysbiosis of Bacteroidetes at phylum level and Flavobacterium at genus level might be primarily responsible for GLY-induced negative growth performance. High throughput targeted quantification for metabolites revealed that GLY changed intestinal metabolites profiles, especially the reduced bile acids and short-chain fatty acids. However, the addition of BC or CB effectively attenuated the adverse effects above by remodeling the gut microbiota composition and improving microbial metabolism. The present study provides novel evidence for ameliorating the harmful effects of GLY on fish species by adding probiotics, which highlights the potential application of probiotics in reducing the health risks of GLY in aquatic environment.
草甘膦（GLY）广泛存在于水生环境中，包括水产养殖系统，对鱼类等水生生物构成了危害。益生菌已被报道可减轻污染物引起的毒性。然而，益生菌是否能降低 GLY 对鱼类的健康风险仍不清楚。本研究通过聚焦两种常用水产养殖益生菌，凝结芽孢杆菌（BC）和丁酸梭菌（CB），来研究 GLY 对鲫鱼（Carassius auratus）的影响。暴露于 GLY 会导致鲫鱼生长迟缓，内脏脂肪和肠道脂肪酶活性降低。16S rRNA 测序表明，门水平的拟杆菌门和属水平的黄杆菌属的微生物失调可能是 GLY 引起负生长性能的主要原因。高通量靶向代谢物定量分析显示，GLY 改变了肠道代谢物谱，特别是降低了胆汁酸和短链脂肪酸。然而，添加 BC 或 CB 通过重塑肠道微生物群落组成和改善微生物代谢，有效地减轻了上述不利影响。本研究为通过添加益生菌来改善 GLY 对鱼类的有害影响提供了新的证据，突出了益生菌在降低水生环境中 GLY 健康风险方面的潜在应用。
