Lu Bingyang, Han Xiao, Zou Dan, Luo Xiao, Liu Li, Wang Jingyue, Maitz Manfred F, Yang Ping, Huang Nan, Zhao Ansha
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu, 610031, China.
School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China.
Mater Today Bio. 2022 Aug 11;16:100392. doi: 10.1016/j.mtbio.2022.100392. eCollection 2022 Dec.
Chronic wounds and the accompanying inflammation are ongoing challenges in clinical treatment. They are usually accompanied by low pH and high oxidative stress environments, limiting cell growth and proliferation. Ordinary medical gauze has limited therapeutic effects on chronic wounds, and there is active research to develop new wound dressings. The chitosan hydrogel could be widely used in biomedical science with great biocompatibility, but the low mechanical properties limit its development. This work uses polyacrylamide to prepare double-network (DN) hydrogels based on bioadhesive catechol-chitosan hydrogels. Cystamine and N, N'-Bis(acryloyl)cystamine, which can be cross-linking agents with disulfide bonds to prepare redox-responsive DN hydrogels and pH-responsive nanoparticles (NPs) prepared by acetalized cyclodextrin (ACD) are used to intelligently release drugs against chronic inflammation microenvironments. The addition of catechol groups and ACD-NPs loaded with the Resolvin E1 (RvE1), promotes cell adhesion and regulates the inflammatory response at the wound site. The preparation of the DN hydrogel in this study can be used to treat and regulate the inflammatory microenvironment of chronic wounds accurately. It provides new ideas for using inflammation resolving factor loaded in DN hydrogel of good biocompatibility with enhanced mechanical properties to intelligent regulate the wound inflammation and promote the wound repaired.
慢性伤口及其伴随的炎症是临床治疗中持续存在的挑战。它们通常伴随着低pH值和高氧化应激环境，限制了细胞的生长和增殖。普通医用纱布对慢性伤口的治疗效果有限，因此人们正在积极研究开发新型伤口敷料。壳聚糖水凝胶具有良好的生物相容性，在生物医学领域有广泛应用，但较低的机械性能限制了其发展。本研究利用聚丙烯酰胺，基于生物粘附性儿茶酚-壳聚糖水凝胶制备双网络（DN）水凝胶。胱胺和N,N'-双(丙烯酰基)胱胺可作为具有二硫键的交联剂制备氧化还原响应性DN水凝胶，由缩醛化环糊精（ACD）制备的pH响应性纳米颗粒（NPs）用于针对慢性炎症微环境智能释放药物。儿茶酚基团的加入以及负载了消退素E1（RvE1）的ACD-NPs，促进了细胞黏附并调节了伤口部位的炎症反应。本研究中DN水凝胶的制备可用于精确治疗和调节慢性伤口的炎症微环境。它为利用具有良好生物相容性且机械性能增强的DN水凝胶中负载的炎症消退因子来智能调节伤口炎症并促进伤口修复提供了新思路。
