Xiang Nan, Zhao Yihan, Wang Siyun, Guo Xinbo
School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, Research Institute for Food Nutrition and Human Health, South China University of Technology, Guangzhou, China.
Department of Food, Nutrition, and Health, University of British Columbia, Vancouver, BC, Canada.
Food Chem (Oxf). 2022 Aug 9;5:100128. doi: 10.1016/j.fochms.2022.100128. eCollection 2022 Dec 30.
The present study aimed to identify the regulatory mechanisms of red, blue, and white light on carotenoid biosynthesis in maize sprouts. Determinations of carotenoid, chlorophyll and phytohormone profiles, as well as relative gene expression, were explored. The results identified enhancement of carotenoid and chlorophyll production as well as gene expression. Most notably, the expression levels of , , and beta-carotene 3-hydroxylase genes peaked under blue light. Photomorphogene-related hormone, auxins and strigolactone production was also altered under different lights and might have a role in carotenoid metabolism. Gibberellins competed with carotenoids for the precursor geranylgeranyl diphosphate and were hindered by certain light characteristics, probably via DELLA-PIF4 signalling. and were negative regulators of carotenoid biosynthesis in maize sprouts. These findings provide new insights into the light-regulated mechanism and biofortification of carotenoids in maize sprouts.
本研究旨在确定红光、蓝光和白光对玉米芽中类胡萝卜素生物合成的调控机制。对类胡萝卜素、叶绿素和植物激素谱以及相关基因表达进行了测定。结果表明类胡萝卜素和叶绿素产量以及基因表达均有所增强。最显著的是，八氢番茄红素合成酶、ζ-胡萝卜素脱氢酶和β-胡萝卜素3-羟化酶基因的表达水平在蓝光下达到峰值。光形态建成相关激素、生长素和独脚金内酯的产生在不同光照下也发生了变化，可能在类胡萝卜素代谢中发挥作用。赤霉素与类胡萝卜素竞争前体香叶基香叶基二磷酸，并受到某些光照特性的阻碍，可能是通过DELLA-PIF4信号通路。八氢番茄红素合成酶和ζ-胡萝卜素脱氢酶是玉米芽中类胡萝卜素生物合成的负调控因子。这些发现为玉米芽中类胡萝卜素的光调控机制和生物强化提供了新的见解。
