Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
Fish Shellfish Immunol. 2022 Nov;130:1-8. doi: 10.1016/j.fsi.2022.08.030. Epub 2022 Aug 26.
Nanoparticles-based treatments is of utmost importance for aquaculture. In this scenario, chitosan-based nanoparticles have been proposed due to the properties of chitosan, which include mucoadhesiveness. Nevertheless, pivotal parameters of chitosan, such as degree of acetylation and molecular weight, are commonly underestimated in the available literature despite the influence they seem to have on the properties of chitosan-based nanoparticles. In this systematic review, the immunomodulator capacity of chitosan nanoparticles used as mucosal vaccines on teleost fish has been evaluated paying special attention to the chitosan properties. Four databases were used for literature search, yielding 486 documents, from which 14 meet the inclusion criteria. Only 21% of the available studies reported properly chitosan properties, which should be improved in future works to generate reproducible data as well as valuable information. To the best of our knowledge, this work objectively compares for the first time, by quantifying the mg of chitosan/g of fish applied in each study, the chitosan nanoparticle preparation and doses applied to fish, as well as the effects of the treatments applied on fish immune status.
基于纳米粒子的治疗方法对水产养殖至关重要。在此背景下，由于壳聚糖具有黏附性等特性，基于壳聚糖的纳米粒子已被提出。然而，尽管壳聚糖的乙酰化程度和分子量等关键参数似乎对壳聚糖基纳米粒子的性质有影响，但在现有文献中，这些参数通常被低估。在这项系统评价中，评估了壳聚糖纳米粒子作为黏附疫苗在硬骨鱼类中的免疫调节能力，特别关注壳聚糖的性质。我们使用了四个数据库进行文献检索，共得到 486 篇文献，其中 14 篇符合纳入标准。只有 21%的现有研究报告了壳聚糖的正确性质，这应该在未来的工作中得到改进，以便生成可重复的数据和有价值的信息。据我们所知，这是首次通过定量比较应用于每种研究的每克鱼的壳聚糖毫克数，客观地比较了用于鱼类的壳聚糖纳米粒子的制备和剂量，以及应用于鱼类的治疗方法对其免疫状态的影响。
