Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, Road Sidi Mansour 6 km, P.O. Box 1177, 3018, Sfax, Tunisia.
Protoplasma. 2023 May;260(3):707-721. doi: 10.1007/s00709-022-01807-5. Epub 2022 Sep 5.
Under field conditions, wheat is subjected to single or multiple stress conditions. The elucidation of the molecular mechanism of stress response is a key step to identify candidate genes for stress resistance in plants. In this study, RNA-seq data analysis identified 17.324, 10.562, 5.510, and 8.653 differentially expressed genes (DEGs) under salt, drought, heat, and cold stress, respectively. Moreover, the comparison of DEGs from each stress revealed 2374 shared genes from which 40% showed highly conserved expression patterns. Moreover, co-expression network analysis and GO enrichment revealed co-expression modules enriched with genes involved in transcription regulation, protein kinase pathway, and genes responding to phytohormones or modulating hormone levels. The expression of 15 selected transcription factor encoding genes was analyzed under abiotic stresses and ABA treatment in durum wheat. The identified transcription factor genes are excellent candidates for genetic engineering of stress tolerance in wheat.
在田间条件下，小麦会受到单一或多种胁迫条件的影响。阐明胁迫响应的分子机制是鉴定植物抗逆候选基因的关键步骤。在这项研究中，通过 RNA-seq 数据分析，分别在盐胁迫、干旱胁迫、热胁迫和冷胁迫下鉴定到 17324、10562、5510 和 8653 个差异表达基因（DEGs）。此外，对每种胁迫下的 DEGs 进行比较，揭示了 2374 个共享基因，其中 40%表现出高度保守的表达模式。此外，共表达网络分析和 GO 富集分析揭示了共表达模块富含参与转录调控、蛋白激酶途径以及响应植物激素或调节激素水平的基因。在硬粒小麦中，分析了 15 个选定的转录因子编码基因在非生物胁迫和 ABA 处理下的表达情况。鉴定到的转录因子基因是小麦抗逆性遗传工程的优秀候选基因。
