Department of Periodontology, State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, School of Stomatology, Fourth Military Medical University, Xi'an, China.
State Key Laboratory of High-Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
Int J Oral Sci. 2022 Sep 5;14(1):45. doi: 10.1038/s41368-022-00195-z.
The positive regulation of bone-forming osteoblast activity and the negative feedback regulation of osteoclastic activity are equally important in strategies to achieve successful alveolar bone regeneration. Here, a molybdenum (Mo)-containing bioactive glass ceramic scaffold with solid-strut-packed structures (Mo-scaffold) was printed, and its ability to regulate pro-osteogenic and anti-osteoclastogenic cellular responses was evaluated in vitro and in vivo. We found that extracts derived from Mo-scaffold (Mo-extracts) strongly stimulated osteogenic differentiation of bone marrow mesenchymal stem cells and inhibited differentiation of osteoclast progenitors. The identified comodulatory effect was further demonstrated to arise from Mo ions in the Mo-extract, wherein Mo ions suppressed osteoclastic differentiation by scavenging reactive oxygen species (ROS) and inhibiting mitochondrial biogenesis in osteoclasts. Consistent with the in vitro findings, the Mo-scaffold was found to significantly promote osteoblast-mediated bone formation and inhibit osteoclast-mediated bone resorption throughout the bone healing process, leading to enhanced bone regeneration. In combination with our previous finding that Mo ions participate in material-mediated immunomodulation, this study offers the new insight that Mo ions facilitate bone repair by comodulating the balance between bone formation and resorption. Our findings suggest that Mo ions are multifunctional cellular modulators that can potentially be used in biomaterial design and bone tissue engineering.
促进成骨细胞活性的正向调节和破骨细胞活性的负反馈调节在实现成功的牙槽骨再生策略中同样重要。在这里，打印了一种含有钼的生物活性玻璃陶瓷支架，具有实体支柱填充结构（Mo 支架），并在体外和体内评估了其调节成骨前体细胞和抗破骨细胞生成细胞反应的能力。我们发现，Mo 支架提取液（Mo 提取物）强烈刺激骨髓间充质干细胞的成骨分化，并抑制破骨细胞前体的分化。鉴定出的协同调节作用源自 Mo 提取物中的 Mo 离子，其中 Mo 离子通过清除活性氧 (ROS) 和抑制破骨细胞中线粒体生物发生来抑制破骨细胞分化。与体外研究结果一致，Mo 支架在整个骨愈合过程中显著促进成骨细胞介导的骨形成并抑制破骨细胞介导的骨吸收，从而增强骨再生。结合我们之前发现的 Mo 离子参与材料介导的免疫调节，本研究提供了新的见解，即 Mo 离子通过调节骨形成和吸收之间的平衡来促进骨修复。我们的研究结果表明，Mo 离子是多功能细胞调节剂，可潜在用于生物材料设计和骨组织工程。
