Tawfik Eman, Ahmed Mohamed Fathy, Albalawi Doha A, Aljuaid Bandar S, Darwish Doaa Bahaa Eldin, Mahmoud Samy F, Hassan Karim M, Ibrahim Mohamed F M, Abdel Razik Ashraf Bakry
Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo 11566, Egypt.
Horticulture Department, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt.
Plants (Basel). 2022 Sep 3;11(17):2311. doi: 10.3390/plants11172311.
Calla lily ( (Hook.) Baill.) is an herbaceous or semi-evergreen perennial grown from rhizomes. It is commonly named "Spotted Arum". Ribosomal RNAs (rRNAs) are found in all known organisms and are known for being functionally equivalent in all of them. A completely new in vitro culture protocol was applied to with two hormones, 6-Benzylaminopurine (BAP) and kinetin, to obtain full growth and multiplication. Due to their highly conserved sequences, the analysis of small-subunit rRNAs (16S-18S rRNAs) can provide precise statistical evaluation of a wide variety of phylogenetic connections. As a result, the plant's 18S rRNA gene allowed for identification and partial sequencing. Also, the traditional floral method and the novel application technique for identification were applied to . In this paper we systemically describe the structural strategies of the plant's adaptation to the surroundings at the morphological, physiological, and anatomical scale. Most the essential oils and fatty acids found in are omega fatty acids, octadecenoic acid, linoleic acid, and palmitic acid. All these fatty acids have industrial, medicinal, and pharmaceutical applications. The significant findings are the spadix sheathing leaves, and the precipitation of raphides calcium oxalate. The mitotic index showing the division activity was recorded, and it was 17.4%. The antimicrobial activity of ethanol extract was performed via the well diffusion method. This extract has shown high activity against and , compared to its lower activity against . By defining these characteristics and in vitro culture conditions, we will be able to acclimatize the plant in greenhouses, and then transfer it to the open field. The findings of this work identified the general characteristics of as an ornamental and medicinal plant in order to acclimatize this plant for cultivation in the Mediterranean climate.
马蹄莲（(Hook.) Baill.）是一种从根茎生长的多年生草本或半常绿植物。它通常被称为“斑叶芋”。核糖体RNA（rRNAs）存在于所有已知生物中，并且在所有生物中功能等同。一种全新的体外培养方案应用于添加两种激素，即6-苄氨基嘌呤（BAP）和激动素，以实现充分生长和增殖。由于其高度保守的序列，小亚基rRNAs（16S - 18S rRNAs）的分析可以为广泛的系统发育联系提供精确的统计评估。因此，该植物的18S rRNA基因得以鉴定和部分测序。此外，还将传统的花卉鉴定方法和新颖的应用技术应用于该植物。在本文中，我们系统地描述了该植物在形态、生理和解剖尺度上适应周围环境的结构策略。马蹄莲中发现的大多数精油和脂肪酸是ω脂肪酸、十八碳烯酸、亚油酸和棕榈酸。所有这些脂肪酸都有工业、药用和制药应用。重要的发现是肉穗花序的鞘叶以及草酸钙针晶的沉淀。记录了显示分裂活性的有丝分裂指数，为17.4%。通过滤纸片扩散法对马蹄莲乙醇提取物进行了抗菌活性测试。与对其他一些菌的较低活性相比，该提取物对某些菌显示出高活性。通过确定这些特征和体外培养条件，我们将能够使该植物适应温室环境，然后转移到露天田地。这项工作的结果确定了马蹄莲作为观赏和药用植物的一般特征，以便使这种植物适应在地中海气候下种植。
