School of Computing Engineering and Mathematical Sciences, La Trobe University, Bendigo, Victoria 3350, Australia.
School of Computing Engineering and Mathematical Sciences, La Trobe University, Bendigo, Victoria 3350, Australia.
Biomater Adv. 2022 Oct;141:213103. doi: 10.1016/j.bioadv.2022.213103. Epub 2022 Sep 2.
The rapid evolution of 3D bioprinting technique, very few biomaterials have been studied and utilised as ink solutions to produce structures. In this work, a polymeric nanocomposite hydrogel-based ink solution was developed using boron nitride nanotubes (BNNTs) reinforced gelatin for 3D bioprinting of scaffolds. The ink solutions and printed scaffolds were characterised for their printability, mechanical, thermal, water uptake, and biological properties (cell viability and inflammation). The viscoelastic behaviour of the scaffolds indicated the increase in storage modulus with an increase in BNNTs composition. Additionally, the compressive strength of the scaffolds increased from 9.43 ± 1.3 kPa to 30.09 ± 1.5 kPa with the addition of BNNTs. Similarly, the thermal stability of the scaffolds enhanced with an increase in BNNTs composition. Furthermore, the scaffolds with a higher concentration of BNNTs displayed resilience in cell culture media at 37 °C for up to 14 days compared with pure gelatin scaffolds. The cell viability results showed a decreased viability rate with an increased concentration of BNNTs scaffolds. However, BNNTs incubated with cells did not display cytokine inflammation. Therefore, this work provides a potential hydrogel-based ink solution for 3D bioprinting of biomimetic tissue constructs with adequate structural stability for a wide range of tissue engineering and regenerative medicine applications.
3D 生物打印技术发展迅速，但作为墨水解决方案用于制造结构的生物材料却寥寥无几。在这项工作中，使用氮化硼纳米管（BNNTs）增强明胶开发了一种基于聚合物纳米复合材料水凝胶的墨水溶液，用于 3D 生物打印支架。对墨水溶液和打印支架进行了可打印性、机械性能、热性能、吸水性和生物性能（细胞活力和炎症）的特性研究。支架的粘弹性行为表明，随着 BNNTs 组成的增加，储能模量增加。此外，随着 BNNTs 的添加，支架的压缩强度从 9.43 ± 1.3 kPa 增加到 30.09 ± 1.5 kPa。同样，随着 BNNTs 组成的增加，支架的热稳定性也得到了提高。此外，与纯明胶支架相比，具有较高 BNNTs 浓度的支架在 37°C 的细胞培养基中具有更高的弹性，可长达 14 天。细胞活力结果表明，随着 BNNTs 支架浓度的增加，细胞活力下降。然而，与细胞共孵育的 BNNTs 并未显示细胞因子炎症。因此，这项工作为 3D 仿生组织构建物的生物打印提供了一种潜在的水凝胶基墨水溶液，具有足够的结构稳定性，适用于广泛的组织工程和再生医学应用。
