Polveiro Richard Costa, Vidigal Pedro Marcus Pereira, de Oliveira Mendes Tiago Antônio, Yamatogi Ricardo Seiti, da Silva Luciana Saraiva, Fujikura Juliana Miwa, Da Costa Mateus Matiuzzi, Moreira Maria Aparecida Scatamburlo
Laboratório de Doenças Bacterianas, Setor de Medicina Veterinária Preventiva e Saúde Pública, Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Núcleo de Análise de Biomoléculas (NuBioMol), Centro de Ciências Biológicas, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Front Microbiol. 2022 Aug 25;13:918706. doi: 10.3389/fmicb.2022.918706. eCollection 2022.
Mastitis, mainly caused by bacterial intramammary infections, is the main problem in the breeding of dairy animals. The inflammations of the mammary gland is separated by types of mastitis, being subclinical, clinical, and the most severe, gangrenous mastitis. Here, we used 16S rRNA amplicon sequencing to characterize the bacterial microbiota of goat milk in the different types of goat mastitis caused by bacteria. We used 72 goat milk samples from a region of the state of Minas Gerais in Brazil, of which 12 were from clinically healthy animals, 42 from animals diagnosed with subclinical mastitis, 16 from animals with clinical mastitis, and 2 from animals with gangrenous mastitis. The group related to gangrenous mastitis was the most divergent in terms of alpha and beta diversity. The most abundant genus among samples of the groups was spp., and we found a high abundance of sp. in the milk of animals diagnosed with clinical mastitis. The most statistically relevant microorganisms among the groups were sp., , sp., and sp. We highlight a new association of bacterial agents in gangrenous mastitis among sp./ sp. and sp. and provide the second report of the genus sp., in milk samples. Only the taxa sp., sp., , and sp., were present in all groups. The superpathway of L-tryptophan biosynthesis metabolites and the sucrose degradation III (sucrose invertase) pathway were the most prominent ones among the groups. In this study, we demonstrate how a rich microbiota of goat milk from healthy animals can be altered during the aggravation of different types of mastitis, in addition to demonstrating new bacterial genera in milk not previously detected in other studies as well as new associations between agents.
乳腺炎主要由乳腺内细菌感染引起，是奶牛养殖中的主要问题。乳腺炎症根据乳腺炎类型分为亚临床型、临床型和最严重的坏疽性乳腺炎。在此，我们使用16S rRNA扩增子测序来表征由细菌引起的不同类型山羊乳腺炎中羊奶的细菌微生物群。我们使用了来自巴西米纳斯吉拉斯州一个地区的72份山羊奶样本，其中12份来自临床健康的动物，42份来自被诊断为亚临床乳腺炎的动物，16份来自患有临床乳腺炎的动物，2份来自患有坏疽性乳腺炎的动物。就α和β多样性而言，与坏疽性乳腺炎相关的组差异最大。各组样本中最丰富的属是 spp.，并且我们在被诊断患有临床乳腺炎的动物的牛奶中发现了高丰度的 sp.。各组中统计学上最相关的微生物是 sp.、 、 sp.和 sp.。我们强调了坏疽性乳腺炎中 sp./ sp.和 sp.之间细菌病原体的新关联，并提供了牛奶样本中 sp.属的第二篇报道。只有 sp.、 sp.、 和 sp.分类群在所有组中都存在。L-色氨酸生物合成代谢物的超级途径和蔗糖降解III（蔗糖转化酶）途径是各组中最突出的途径。在本研究中，我们展示了健康动物丰富的羊奶微生物群在不同类型乳腺炎加重过程中是如何变化的，此外还展示了牛奶中以前在其他研究中未检测到的新细菌属以及病原体之间的新关联。
