Wang Dan-Ni, Feng Jie, Yu Chen-Xi, Zhang Xin-Kai, Chen Jun, Wei Liu-Jing, Liu Zhijie, Ouyang Liming, Zhang Lixin, Hua Qiang, Liu Feng
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China.
Key Laboratory of Fermentation Engineering (Ministry of Education), Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China.
Synth Syst Biotechnol. 2022 Aug 18;7(4):1133-1141. doi: 10.1016/j.synbio.2022.08.001. eCollection 2022 Dec.
Astaxanthin is a high value carotenoid with a broad range of commercial applications due to its superior antioxidant properties. In this study, β-carotene-producing XK17 constructed in the lab was employed for astaxanthin biosynthesis. The catalytic effects of β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ from various species were investigated. The PspCrtW from sp. and HpCrtZ from were confirmed to be the best combination in converting β-carotene. Several key bottlenecks in biomass and astaxanthin biosynthesis were effectively eliminated by optimizing the expression of the above enzymes and restoring uracil/leucine biosynthesis. In addition, the effects of astaxanthin biosynthesis on cell metabolism were investigated by integrated analysis of pathway modification and transcriptome information. After further optimization, strain DN30 was able to synthesize up to 730.3 mg/L astaxanthin in laboratory 5-L fermenter. This study provides a good metabolic strategy and a sustainable development platform for high-value carotenoid production.
虾青素是一种高价值类胡萝卜素，因其卓越的抗氧化特性而具有广泛的商业应用。在本研究中，实验室构建的产β-胡萝卜素的XK17被用于虾青素的生物合成。研究了来自不同物种的β-胡萝卜素酮酶CrtW和β-胡萝卜素羟化酶CrtZ的催化效果。来自某物种的PspCrtW和来自另一物种的HpCrtZ被证实是转化β-胡萝卜素的最佳组合。通过优化上述酶的表达并恢复尿嘧啶/亮氨酸生物合成，有效消除了生物量和虾青素生物合成中的几个关键瓶颈。此外，通过途径修饰和转录组信息的综合分析，研究了虾青素生物合成对细胞代谢的影响。经过进一步优化，菌株DN30在实验室5升发酵罐中能够合成高达730.3毫克/升的虾青素。本研究为高价值类胡萝卜素的生产提供了良好的代谢策略和可持续发展平台。
