Morozova Maryana V, Kalmykova Galina V, Akulova Nadezhda I, Ites Yuriy V, Korkina Valentina I, Litvinova Ekaterina A
Scientific-Research Institute of Neurosciences and Medicine, St. Timakova, 4, 630117 Novosibirsk, Russia.
Physical Engineering Faculty, Novosibirsk State Technical University, 630073 Novosibirsk, Russia.
Animals (Basel). 2022 Sep 13;12(18):2399. doi: 10.3390/ani12182399.
Within barrier facilities, autoclaved diet and bedding are used for husbandry of laboratory rodents. spp. are ubiquitous in nature and some of them are known as probiotics. Inactivation of the spores and reduction of the diet nutritional value due to autoclavation could be especially critical for immunodeficient mice. We studied the effect of the autoclaved and non-autoclaved diets on the reproductive performance and the age of prolapse manifestation in mice with impaired gut barrier function and, therefore, sensitive to change of microbiota. We found that the non-autoclaved diet led to enhancement of the fertility index of and female mice. The non-autoclaved diet affected the prolapse of mice that occurred later in comparison with females eating the autoclaved diet. We showed that spp. was present in the non-autoclaved diet and feces of mice on the non-autoclaved diet. Bacterial strains of the non-autoclaved diet and feces belonged to , , , , , and other representatives of spp. Moreover, autoclavation of the diet affected on the percent of the blood and spleen immune cells, the bacterial composition of the intestine, and increased the level of methionine in the thigh muscle of mice. Enhanced reproductive performance and delayed prolapse manifestation in mice could be due to improved digestion, as spp. from diet and feces had enzymatic activity.
在屏障设施内，高压灭菌的饲料和垫料用于饲养实验啮齿动物。某些菌属在自然界中普遍存在，其中一些被称为益生菌。对于免疫缺陷小鼠而言，高压灭菌导致的孢子失活和饲料营养价值降低可能尤为关键。我们研究了高压灭菌饲料和未高压灭菌饲料对肠道屏障功能受损、因此对微生物群变化敏感的小鼠的繁殖性能和子宫脱垂表现年龄的影响。我们发现，未高压灭菌的饲料提高了雌性小鼠的生育指数。与食用高压灭菌饲料的雌性小鼠相比，未高压灭菌的饲料使小鼠子宫脱垂出现得更晚。我们发现，未高压灭菌的饲料和食用该饲料的小鼠粪便中存在某些菌属。未高压灭菌的饲料和粪便中的细菌菌株属于某些菌属的、、、、、及其他代表菌。此外，饲料的高压灭菌影响了血液和脾脏免疫细胞的百分比、肠道细菌组成，并提高了小鼠大腿肌肉中甲硫氨酸的水平。小鼠繁殖性能的提高和子宫脱垂表现的延迟可能是由于消化改善，因为饲料和粪便中的某些菌属具有酶活性。
