Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, Araras 13600-970, SP, Brazil.
ADB Pesquisa e Desenvolvimento, Araras 13600-140, SP, Brazil.
Biosensors (Basel). 2022 Sep 16;12(9):761. doi: 10.3390/bios12090761.
Silver materials are known to present excellent properties, such as high electrical and thermal conductivity as well as chemical stability. Silver-based inks have drawn a lot of attention for being compatible with various substrates, which can be used in the production uniform and stable pseudo-reference electrodes with low curing temperatures. Furthermore, the interest in the use of disposable electrodes has been increasing due to the low cost and the possibility of their use in point-of-care and point-of-need situations. Thus, in this work, two new inks were developed using Ag as conductive material and colorless polymers (nail polish (NP) and shellac (SL)), and applied to different substrates (screen-printed electrodes, acetate sheets, and 3D-printed electrodes) to verify the performance of the proposed inks. Measurements attained with open circuit potential (OCP) attested to the stability of the potential of the pseudo-reference proposed for 1 h. Analytical curves for β-estradiol were also obtained using the devices prepared with the proposed inks as pseudo-references electrodes, which presented satisfactory results concerning the potential stability (RSD < 2.6%). These inks are simple to prepare and present great alternatives for the development of pseudo-reference electrodes useful in the construction of disposable electrochemical systems.
银材料具有优良的性能，如高导电性和导热性以及化学稳定性。银基油墨因其与各种基底兼容而受到广泛关注，可用于生产具有低固化温度的均匀稳定的赝参考电极。此外，由于成本低且可用于即时护理和即时需求情况下，对使用一次性电极的兴趣日益增加。因此，在这项工作中，使用 Ag 作为导电材料和无色聚合物（指甲油（NP）和虫胶（SL））开发了两种新的油墨，并应用于不同的基底（丝网印刷电极、醋酸盐片和 3D 打印电极），以验证所提出油墨的性能。开路电位 (OCP) 的测量证明了所提出的赝参考电极的电位稳定性在 1 小时内保持稳定。使用所提出的油墨作为伪参考电极制备的设备还获得了β-雌二醇的分析曲线，这些设备在电位稳定性方面呈现出令人满意的结果（RSD<2.6%）。这些油墨易于制备，是开发用于构建一次性电化学系统的赝参考电极的理想选择。
