School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Joint International Research Laboratory of Agriculture Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, China.
J Agric Food Chem. 2022 Oct 12;70(40):12733-12745. doi: 10.1021/acs.jafc.2c04238. Epub 2022 Sep 27.
The food-gut microbiota interaction is an important regulator of human health. Numerous in vitro and animal models have thus been developed in order to simulate the specific food-gut microbiota and/or host-gut microbiota interactions in the human colon. This review summarizes the design principles of each model and discusses their advantages and weaknesses in terms of studying food-gut microbiota interactions. In vitro fermentation models appear to be reliable methods to investigate various aspects involved in the food-gut microbiota interactions in humans. However, many physiological perspectives lack appreciation of these models, such as peristaltic movement, biochemical conditions, and gastrointestinal anatomy. Animal models provide more physiological relevance to human trials compared to in vitro models. However, they may have gastrointestinal tract aspects that are distinct from human subjects. This review contains important information that can help the development of more advanced models to study food-gut microbiota interactions in humans.
食物-肠道微生物群相互作用是人类健康的重要调节剂。因此，已经开发了许多体外和动物模型，以模拟人类结肠中特定的食物-肠道微生物群和/或宿主-肠道微生物群相互作用。本综述总结了每种模型的设计原则，并讨论了它们在研究食物-肠道微生物群相互作用方面的优缺点。体外发酵模型似乎是研究人类食物-肠道微生物群相互作用各个方面的可靠方法。然而，许多生理观点并没有充分认识到这些模型，例如蠕动运动、生化条件和胃肠道解剖结构。与体外模型相比，动物模型为人体试验提供了更多的生理相关性。然而，它们的胃肠道方面可能与人类受试者不同。本综述包含了有助于开发更先进的模型来研究人类食物-肠道微生物群相互作用的重要信息。
