Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, P.R.China.
Pol J Microbiol. 2022 Sep 24;71(3):421-428. doi: 10.33073/pjm-2022-037. eCollection 2022 Sep 1.
Phage P2 was isolated from failed fermentation broth carried out by IMAU10120. A previous study in our laboratory showed that this phage belonged to the family. In this study, this phage's genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.
噬菌体 P2 是从 IMAU10120 发酵失败的发酵液中分离出来的。我们实验室的先前研究表明，这种噬菌体属于 科。在本研究中，通过全基因组测序分析了该噬菌体的基因组特征。结果表明，噬菌体 P2 的长度为 77.9 kb，GC 含量为 39.28%。其基因组包含 96 个编码序列（CDS）和两个 tRNA 基因，参与结构、DNA 复制、包装和调控的功能。噬菌体 P2 具有更高的宿主特异性；许多测试菌株未被感染。细胞壁吸附实验表明，噬菌体 P2 的吸附受体成分可能是细胞壁肽聚糖的一部分。这项研究可能丰富了乳酸菌噬菌体基因组信息的知识，并为建立噬菌体控制措施提供了一些初步数据。
