Li Jianhua, Wang Fang, Cui Yunhua, Song Li, Yu Yongmei, Chen Xia
Department of Otolaryngology, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, China.
Department of Otolaryngology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
Evid Based Complement Alternat Med. 2022 Sep 28;2022:6153577. doi: 10.1155/2022/6153577. eCollection 2022.
In this study, 383 differentially expressed genes (DEGs) between the allergic group and the nonallergic group were excavated. Humoral immune response, chemokine-related biological processes, granulocyte-related biological processes, IL-17 signaling pathway, and TNF signaling pathway were found connected with DEGs. The allergic group had significantly higher enrich scores of T cells, T helper cells, TFH, and Th17 cells than the nonallergic group. We acquired 26 rape pollen allergy-associated genes by taking the intersection of key module genes from WGCNA and the DEGs. The functional enrichment results show that rape pollen allergy-associated genes are relevant to processes and pathways like regulation of inflammatory response, transcriptional regulation, lymphocyte differentiation, IL-17 signaling pathway, and MAPK signaling pathway. Then, three characteristic genes were defined by crossing the genes derived from LASSO and SVM-RFE algorithms, including , 1, and 1. The AUC values of these genes manifested that the three genes had a mighty discrimination power in discriminating allergic samples from nonallergic samples. In conclusion, this study revealed three characteristic genes (, 1, and 1) in rape pollen allergy, suggesting that they may be potential biomarkers in rape pollen allergy diagnosis and treatment.
在本研究中，挖掘出了过敏组和非过敏组之间的383个差异表达基因（DEGs）。发现体液免疫反应、趋化因子相关生物学过程、粒细胞相关生物学过程、IL-17信号通路和TNF信号通路与DEGs相关。过敏组的T细胞、辅助性T细胞、滤泡辅助性T细胞和Th17细胞的富集分数显著高于非过敏组。通过取加权基因共表达网络分析（WGCNA）的关键模块基因与DEGs的交集，我们获得了26个与油菜花粉过敏相关的基因。功能富集结果表明，油菜花粉过敏相关基因与炎症反应调节、转录调节、淋巴细胞分化、IL-17信号通路和丝裂原活化蛋白激酶（MAPK）信号通路等过程和通路相关。然后，通过交叉源自套索（LASSO）和支持向量机递归特征消除（SVM-RFE）算法的基因定义了三个特征基因，包括 、1和1。这些基因的曲线下面积（AUC）值表明，这三个基因在区分过敏样本和非过敏样本方面具有强大的判别能力。总之，本研究揭示了油菜花粉过敏中的三个特征基因（ 、1和1），表明它们可能是油菜花粉过敏诊断和治疗中的潜在生物标志物。
