Zhu Ling, Andersen-Civil Audrey I S, Castro-Meija Josue L, Nielsen Dennis S, Blanchard Alexandra, Olsen John E, Thamsborg Stig M, Williams Andrew R
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark.
Department of Food Science, University of Copenhagen, 1958 Frederiksberg, Denmark.
Antioxidants (Basel). 2022 Oct 15;11(10):2033. doi: 10.3390/antiox11102033.
The garlic-derived compounds propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO) are metabolites with putative health benefits against intestinal inflammation that may be related to their antioxidant activity. However, the underlying mechanisms remain unclear, and whether PTS-PTSO can promote gut health by altering the microbiota and exert protection against enteric pathogens needs further investigation. Here, we explored the antioxidant activity of PTS-PTSO in murine macrophages in vitro, and in an in vivo model of bacterial infection with the bacterial pathogen . PTS-PTSO attenuated reactive oxygen species in lipopolysaccharide-stimulated macrophages in a nuclear factor erythroid factor 2-related factor 2 (Nrf2)-dependent manner, decreased nitric oxide levels both in macrophages in vitro and in the sera of mice fed PTS-PTSO, and had putatively beneficial effects on the commensal gut microbiota. Importantly, PTS-PTSO decreased faecal counts, concomitant with upregulation of Nrf2-related genes in colon tissue. Thus, PTS-PTSO mediates Nrf2-mediated antioxidant activity and modulates gut microbiota, which may protect the host against colonization. Our results provide further insight into how PTS-PTSO and related bioactive dietary compounds may reduce enteric infections.
大蒜衍生化合物丙基丙烷硫代亚磺酸盐（PTS）和丙基丙烷硫代磺酸盐（PTSO）是具有潜在健康益处的代谢产物，可能与其抗氧化活性有关，对肠道炎症有抵御作用。然而，其潜在机制仍不清楚，PTS - PTSO是否能通过改变微生物群促进肠道健康并抵御肠道病原体尚需进一步研究。在此，我们在体外小鼠巨噬细胞以及细菌病原体感染的体内模型中探究了PTS - PTSO的抗氧化活性。PTS - PTSO以依赖核因子红细胞2相关因子2（Nrf2）的方式减弱脂多糖刺激的巨噬细胞中的活性氧，降低体外巨噬细胞和喂食PTS - PTSO的小鼠血清中的一氧化氮水平，并且对共生肠道微生物群有潜在的有益影响。重要的是，PTS - PTSO减少了粪便细菌数量，同时结肠组织中Nrf2相关基因上调。因此，PTS - PTSO介导Nrf2介导的抗氧化活性并调节肠道微生物群，这可能保护宿主免受细菌定植。我们的结果进一步深入了解了PTS - PTSO和相关生物活性膳食化合物如何减少肠道感染。
