Darwish Ahmed M, Mabrouk Dalia M, Desouky Hassan M, Khattab Abd El-Nasser
Cell Biology Department, Biotechnology Research Institute, National Research Centre, DokkiGiza, 12622, Egypt.
Animal Reproduction and Artificial Insemination Department, Veterinary Research Institute, National Research Centre, Dokki, Giza, Egypt.
J Genet Eng Biotechnol. 2022 Oct 27;20(1):148. doi: 10.1186/s43141-022-00427-z.
Kidney disease (KD) is a public health problem worldwide and is an important factor in peripheral vascular disease, arrhythmias, heart failure, acute myocardial infarction, stroke, and angina. Obesity has been indicated as an effective cause of kidney diseases. So, this study aims to use two new strains of Lactobacillus to reduce the metabolic disorders and kidney insufficiency associated with obesity.
Fifty BALB/c male mice were divided into five groups (control, obesity, obesity pro1, obesity pro2, and obesity mix). The bodyweight, cholesterol profile, urea, and creatinine levels in urine and serum were all measured. Histopathological analysis and expression of Opn, Vim, Ngal, Kim-1, and αKlotho genes for kidney tissues were performed.
The results indicated that body weight, cholesterol profile, urea, and creatinine levels in serum and urine had the lowest significance (P ˂ 0.05) in the obesity mix group and the highest significance in the obesity group. HDL had the highest significance (P ˂ 0.05) in the obesity mix group and the lowest significance (P ˂ 0.05) in the obesity group. Expression of Opn, Vim, Ngal, and Kim-1 genes was the most upregulated in the obesity group compared with the other groups, and there were nonsignificant differences (P > 0.05) between the obesity pro1 and obesity mix groups and the control group. Expression of αKlotho gene was significantly reduced (P ˂ 0.05) in the obesity group compared with the control group.
This study demonstrated that the combination of pro1 and pro2 strains could reduce kidney inflammation and necrosis.
肾脏疾病（KD）是一个全球性的公共卫生问题，是外周血管疾病、心律失常、心力衰竭、急性心肌梗死、中风和心绞痛的重要因素。肥胖已被指出是肾脏疾病的一个有效病因。因此，本研究旨在使用两种新型乳酸杆菌菌株来减少与肥胖相关的代谢紊乱和肾功能不全。
将50只BALB/c雄性小鼠分为五组（对照组、肥胖组、肥胖pro1组、肥胖pro2组和肥胖混合组）。测量体重、胆固醇水平、尿液和血清中的尿素及肌酐水平。对肾脏组织进行组织病理学分析以及Opn、Vim、Ngal、Kim-1和αKlotho基因的表达检测。
结果表明，血清和尿液中的体重、胆固醇水平、尿素及肌酐水平在肥胖混合组中具有最低的显著性（P＜0.05），在肥胖组中具有最高的显著性。高密度脂蛋白（HDL）在肥胖混合组中具有最高的显著性（P＜0.05），在肥胖组中具有最低的显著性（P＜0.05）。与其他组相比，肥胖组中Opn、Vim、Ngal和Kim-1基因的表达上调最为明显，肥胖pro1组、肥胖混合组与对照组之间无显著差异（P＞0.05）。与对照组相比，肥胖组中αKlotho基因的表达显著降低（P＜0.05）。
本研究表明，pro1和pro2菌株的组合可减轻肾脏炎症和坏死。
